Biophysical Journal 72: 997-1005 (1997)
© 1997 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yonekura, K Articles by Toyoshima, C Search for Related Content PubMed PubMed Citation Articles by Yonekura, K Articles by Toyoshima, C

The ATP-binding site of Ca(2+)-ATPase revealed by electron image analysis.

Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.

ABSTRACT

The location of the ATP-binding site of a P-type ion pump, Ca(2+)-ATPase from rabbit sarcoplasmic reticulum, was examined by cryoelectron microscopy. A nonhydrolyzable analog of ATP, beta, gamma-bidentate chromium (III) complex of ATP (CrATP), was used to stabilize the enzyme in the Ca(2+)-occluded state. Tubular crystals were then induced by vanadate in the presence of EGTA, keeping CrATP bound to the enzyme. The three-dimensional structures of the crystals were determined at 14 A resolution by cryoelectron microscopy and helical image analysis. Statistical comparison of the structures with and without CrATP showed clear and significant differences at the groove proposed previously as the ATP-binding pocket.

This article has been cited by other articles:

				G. Scarborough Crystallization, structure and dynamics of the proton-translocating P-type ATPase J. Exp. Biol., January 1, 2000; 203(1): 147 - 154. [Abstract]

				D. Seto-Young, M. Bandell, M. Hall, and D. S. Perlin Differential Exposure of Surface Epitopes in the beta -Strand Region of LOOP1 of the Yeast H+-ATPase during Catalysis J. Biol. Chem., July 17, 1998; 273(29): 18282 - 18287. [Abstract] [Full Text] [PDF]

				P. Champeil, T. Menguy, S. Soulie, B. Juul, A. G. de Gracia, F. Rusconi, P. Falson, L. Denoroy, F. Henao, M. le Maire, et al. Characterization of a Protease-resistant Domain of the Cytosolic Portion of Sarcoplasmic Reticulum Ca2+-ATPase. NUCLEOTIDE- AND METAL-BINDING SITES J. Biol. Chem., March 20, 1998; 273(12): 6619 - 6631. [Abstract] [Full Text] [PDF]

				T. Sorensen, B. Vilsen, and J. P. Andersen Mutation Lys758 right-arrow Ile of the Sarcoplasmic Reticulum Ca2+-ATPase Enhances Dephosphorylation of E2P and Inhibits the E2 to E1Ca2 Transition J. Biol. Chem., November 28, 1997; 272(48): 30244 - 30253. [Abstract] [Full Text] [PDF]

				D. H. MacLennan, W. J. Rice, and N. M. Green The Mechanism of Ca2+ Transport by Sarco(Endo)plasmic Reticulum Ca2+-ATPases J. Biol. Chem., November 14, 1997; 272(46): 28815 - 28818. [Full Text] [PDF]