Biophysical Journal 72: 2180-2186 (1997)
© 1997 the Biophysical Society

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Phosphatydylglycerol promotes bilayer insertion of salmon calcitonin.

Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Sciences, University of Edinburgh, Summerhall, Scotland. j.bradshaw@ed.ac.uk

ABSTRACT

Neutron diffraction from oriented multibilayers has been used to study the bilayer interaction of the amphipathic peptide salmon calcitonin. Penetration of calcitonin into bilayers composed of dioleoylphosphatidylcholine increases with the addition of 15% (mol) of the anionic phospholipid dioleoylphosphatidylglycerol. Neutron scattering profiles of water distribution in stacked bilayers show a continuous band of deuterons across each bilayer, consistent with the suggestion that the hormone forms transbilayer alpha-helixes under these conditions. These experiments add to the growing body of data on the role of phosphatidylglycerol in bilayer insertion of protein helices and suggests a possible evolutionary history for calcitonin.

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