| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 73: 428-445 (1997)
© 1997 the Biophysical Society
Laboratory of Experimental Cardiac Pathology, Cardiology Research Center, Moscow, Russia.
ABSTRACT
The mathematical model of the compartmentalized energy transfer system in cardiac myocytes presented includes mitochondrial synthesis of ATP by ATP synthase, phosphocreatine production in the coupled mitochondrial creatine kinase reaction, the myofibrillar and cytoplasmic creatine kinase reactions, ATP utilization by actomyosin ATPase during the contraction cycle, and diffusional exchange of metabolites between different compartments. The model was used to calculate the changes in metabolite profiles during the cardiac cycle, metabolite and energy fluxes in different cellular compartments at high workload (corresponding to the rate of oxygen consumption of 46 mu atoms of O.(g wet mass)-1.min-1) under varying conditions of restricted ADP diffusion across mitochondrial outer membrane and creatine kinase isoenzyme "switchoff." In the complete system, restricted diffusion of ADP across the outer mitochondrial membrane stabilizes phosphocreatine production in cardiac mitochondria and increases the role of the phosphocreatine shuttle in energy transport and respiration regulation. Selective inhibition of myoplasmic or mitochondrial creatine kinase (modeling the experiments with transgenic animals) results in "takeover" of their function by another, active creatine kinase isoenzyme. This mathematical modeling also shows that assumption of the creatine kinase equilibrium in the cell may only be a very rough approximation to the reality at increased workload. The mathematical model developed can be used as a basis for further quantitative analyses of energy fluxes in the cell and their regulation, particularly by adding modules for adenylate kinase, the glycolytic system, and other reactions of energy metabolism of the cell.
This article has been cited by other articles:
 |
|
 |
|
  V. A. Selivanov, S. Krause, J. Roca, and M. Cascante Modeling of Spatial Metabolite Distributions in the Cardiac Sarcomere Biophys. J., May 15, 2007; 92(10): 3492 - 3500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Balaban Modeling mitochondrial function Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1107 - C1113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Saks, P. Dzeja, U. Schlattner, M. Vendelin, A. Terzic, and T. Wallimann Cardiac system bioenergetics: metabolic basis of the Frank-Starling law J. Physiol., March 1, 2006; 571(2): 253 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Kinsey, P. Pathi, K. M. Hardy, A. Jordan, and B. R. Locke Does intracellular metabolite diffusion limit post-contractile recovery in burst locomotor muscle? J. Exp. Biol., July 15, 2005; 208(14): 2641 - 2652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vendelin, M. Lemba, and V. A. Saks Analysis of Functional Coupling: Mitochondrial Creatine Kinase and Adenine Nucleotide Translocase Biophys. J., July 1, 2004; 87(1): 696 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ventura-Clapier, A. Garnier, and V. Veksler Energy metabolism in heart failure J. Physiol., February 15, 2004; 555(1): 1 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Andrienko, A. V. Kuznetsov, T. Kaambre, Y. Usson, A. Orosco, F. Appaix, T. Tiivel, P. Sikk, M. Vendelin, R. Margreiter, et al. Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells J. Exp. Biol., June 15, 2003; 206(12): 2059 - 2072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Saks, A. Kuznetsov, T. Andrienko, Y. Usson, F. Appaix, K. Guerrero, T. Kaambre, P. Sikk, M. Lemba, and M. Vendelin Heterogeneity of ADP Diffusion and Regulation of Respiration in Cardiac Cells Biophys. J., May 1, 2003; 84(5): 3436 - 3456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Joubert, J.-L. Mazet, P. Mateo, and J. A. Hoerter 31P NMR Detection of Subcellular Creatine Kinase Fluxes in the Perfused Rat Heart. CONTRACTILITY MODIFIES ENERGY TRANSFER PATHWAYS J. Biol. Chem., May 17, 2002; 277(21): 18469 - 18476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K Aliev, P. Dos Santos, J. A Hoerter, S. Soboll, A. N Tikhonov, and V. A Saks Water content and its intracellular distribution in intact and saline perfused rat hearts revisited Cardiovasc Res, January 1, 2002; 53(1): 48 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vendelin, O. Kongas, and V. Saks Regulation of mitochondrial respiration in heart cells analyzed by reaction-diffusion model of energy transfer Am J Physiol Cell Physiol, April 1, 2000; 278(4): C747 - C764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. G. M. van Beek, M. H. van Wijhe, M. H. J. Eijgelshoven, and J. B. Hak Dynamic adaptation of cardiac oxidative phosphorylation is not mediated by simple feedback control Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1375 - H1384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mateo, V. Stepanov, B. Gillet, J.-C. Beloeil, and J. A. Hoerter Cardiac performance and creatine kinase flux during inhibition of ATP synthesis in the perfused rat heart Am J Physiol Heart Circ Physiol, July 1, 1999; 277(1): H308 - H317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Saupe, M. Spindler, R. Tian, and J. S. Ingwall Impaired Cardiac Energetics in Mice Lacking Muscle-Specific Isoenzymes of Creatine Kinase Circ. Res., May 4, 1998; 82(8): 898 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Saupe, M. Spindler, J. C. A. Hopkins, W. Shen, and J. S. Ingwall Kinetic, Thermodynamic, and Developmental Consequences of Deleting Creatine Kinase Isoenzymes from the Heart. REACTION KINETICS OF THE CREATINE KINASE ISOENZYMES IN THE INTACT HEART J. Biol. Chem., June 23, 2000; 275(26): 19742 - 19746. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
