Biophysical Journal 74: 328-337 (1998)
© 1998 the Biophysical Society

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Biophys J, January 1998, p. 328-337, Vol. 74, No. 1

Gramicidin Channel Kinetics under Tension

 ^*Center for Studies in Physics and Biology, Rockefeller University, New York, New York 10021;  ^#NEC Research Institute, Princeton, New Jersey 08540; and  ^§Department of Physiology and Biophysics, Cornell University Medical College, New York, New York 10021 USA

We have measured the effect of tension on dimerization kinetics of the channel-forming peptide gramicidin A. By aspirating large unilamellar vesicles into a micropipette electrode, we are able to simultaneously monitor membrane tension and electrical activity. We find that the dimer formation rate increases by a factor of 5 as tension ranges from 0 to 4 dyn/cm. The dimer lifetime also increases with tension. This behavior is well described by a phenomenological model of membrane elasticity in which tension modulates the mismatch in thickness between the gramicidin dimer and membrane.

Biophys J, January 1998, p. 328-337, Vol. 74, No. 1
© 1998 by the Biophysical Society   0006-3495/98/01/328/10  $2.00

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