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Biophys J, February 1998, p. 892-898, Vol. 74, No. 2

Change of Motion and Localization of Cholesterol Molecule during Lalpha -HII Transition

Eri Hayakawa,* Mutsuo Naganuma,# Kôichi Mukasa,* Tateo Shimozawa, and Tsunehisa Araiso§

 *Nanoelectronics Laboratory, Graduate School of Engineering;  #Department of Neurological Medicine;  Laboratory of Neuro Cybernetics, Research Institute for Electronic Science; and  §Center for Advanced Science and Technology, Hokkaido University, Sapporo 060, Japan

Formation of the inverted hexagonal (HII) phase from the lamellar (Lalpha ) phase of bovine brain-extracted phosphatidylcholine (BBPC) and phosphatidylethanolamine (BBPE) was investigated using 31P-NMR with or without cholesterol. When the ratio of BBPC to BBPE was 1:1, the HII formation was observed in the presence of 33 mol% cholesterol (i.e., BBPC:BBPE:cholesterol = 1:1:1) at 47°C. The fraction of the HII phase in the BBPC/BBPE/cholesterol system could be controlled by the addition of dioleoylglycerol. The change of molecular motion of cholesterol affected by the HII formation was measured at various ratios of the Lalpha to HII phase with the time-resolved fluorescence depolarization method, using dehydroergosterol as a fluorescent probe. It is observed that the motion of cholesterol became vigorous in the mixture state of the Lalpha and the HII phases compared to that in the Lalpha or the HII phase only. These facts show that cholesterol has the strong ability to induce the HII phase, probably by special molecular motion, which includes change of its location from the headgroup area to the acyl-chain area.

Biophys J, February 1998, p. 892-898, Vol. 74, No. 2
© 1998 by the Biophysical Society   0006-3495/98/02/892/07  $2.00


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