help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lambert, O.
Right arrow Articles by Rigaud, J.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lambert, O.
Right arrow Articles by Rigaud, J.-L.

Biophys J, February 1998, p. 918-930, Vol. 74, No. 2

A New "Gel-like" Phase in Dodecyl Maltoside-Lipid Mixtures: Implications in Solubilization and Reconstitution Studies

Olivier Lambert,* Daniel Levy,* Jean-Luc Ranck,# Gerard Leblanc,§ and Jean-Louis Rigaud*

 *Institut Curie, Section de Recherche, UMR-CNRS168 and LCR-CEA 8, 75231 Paris Cedex;  #Centre de Génétique Moléculaire, CNRS, 91190 Gif sur Yvette; and  §Laboratoire Jean Maetz, CEA, 06230 Villefranche-sur-Mer, France

The interaction of dodecyl maltoside with lipids was investigated through the studies of solubilization and reconstitution processes. The solubilization of large unilamellar liposomes was analyzed through changes in turbidity and cryo-transmission electron microscopy. Solubilization was well described by the three-stage model previously reported for other detergents, and the critical detergent/phospholipid ratios at which lamellar-to-micellar transition occurred (Rsat = 1 mol/mol) and finished (Rsol = 1.6 mol/mol) were determined. The vesicle-micelle transition was further observed in the vitrified hydrated state by cryo-transmission electron microscopy. A striking feature of the solubilization process by dodecyl maltoside was the discovery of a new phase consisting of a very viscous "gel-like" sample. It is shown that this equilibrium cohesive phase is composed of long filamentous thread-like micelles, over microns in length. Similar structures were observed upon solubilization of sonicated liposomes, multilamellar liposomes, or biological Ca2+ ATPase membranes. This "gel-like" phase was also visualized during the process of liposome reconstitution after detergent removal from lipid-dodecyl maltoside micelles. The rate of detergent removal, controlled through the use of SM2 Bio-Beads, was demonstrated to drastically influence the morphology of reconstituted liposomes with a propensity for multilamellar liposome formation upon slow transition through the "gel-like" phase. Finally, on the basis of these observations, the mechanisms of dodecyl maltoside-mediated reconstitution of bacteriorhodopsin were analyzed, and optimal conditions for reconstitution were defined.

Biophys J, February 1998, p. 918-930, Vol. 74, No. 2
© 1998 by the Biophysical Society   0006-3495/98/02/918/13  $2.00


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. S. Funari, B. Nuscher, G. Rapp, and K. Beyer
Detergent-phospholipid mixed micelles with a crystalline phospholipid core
PNAS, July 31, 2001; 98(16): 8938 - 8943.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. M. Garavito and S. Ferguson-Miller
Detergents as Tools in Membrane Biochemistry
J. Biol. Chem., August 24, 2001; 276(35): 32403 - 32406.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Biophysical Society.