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Biophys J, March 1998, p. 1333-1345, Vol. 74, No. 3

Functional Co-assembly among Subunits of Cyclic-Nucleotide-Activated, Nonselective Cation Channels, and Across Species from Nematode to Human

J. T. Finn,* D. Krautwurst,#§ J. E. Schroeder,# T.-Y. Chen,* R. R. Reed,*#§ and K.-W. Yau*#

 *Department of Neuroscience,  #Howard Hughes Medical Institute, and Departments of  §Molecular Biology and Genetics and  Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 USA

Cyclic-nucleotide-activated, nonselective cation channels have a central role in sensory transduction. They are most likely tetramers, composed of two subunits (alpha  and beta  or 1 and 2), with the former, but not the latter, being able to form homomeric cyclic-nucleotide-activated channels. Identified members of this channel family now include, in vertebrates, the rod and cone channels mediating visual transduction and the channel mediating olfactory transduction, each apparently with distinct alpha - and beta -subunits. Homologous channels have also been identified in Drosophila melanogaster and Caenorhabditis elegans. By co-expressing any combination of two alpha -subunits, or alpha - and beta -subunits, of this channel family in HEK 293 cells, we have found that they can all co-assemble functionally with each other, including those from fly and nematode. This finding suggests that the subunit members so far identified form a remarkably homogeneous and conserved group, functionally and evolutionarily, with no subfamilies yet identified. The ability to cross-assemble allows these subunits to potentially generate a diversity of heteromeric channels, each with properties specifically suited to a particular cellular function.

Biophys J, March 1998, p. 1333-1345, Vol. 74, No. 3
© 1998 by the Biophysical Society   0006-3495/98/03/1333/13  $2.00


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