help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, W.
Right arrow Articles by Schwartz, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, W.
Right arrow Articles by Schwartz, D. C.

Biophys J, July 1998, p. 513-520, Vol. 75, No. 1

Scanning Force Microscopy of DNA Molecules Elongated by Convective Fluid Flow in an Evaporating Droplet

Weining Wang, Jieyi Lin, and David C. Schwartz

W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, New York, New York 10003 USA

Scanning force microscopy (SFM) was used to image intact, nearly fully elongated lambda bacteriophage DNA molecules, fixed onto freshly cleaved mica surfaces. Molecular elongation and fixation were accomplished using a newly characterized fixation technique, termed "fluid fixation." Here convective fluid flows generated within an evaporating droplet of DNA solution efficiently elongate DNA molecules for fixation onto suitably charged surfaces. SFM images of a very large bacteriophage genome, G, showed the presence of double-stranded bubbles. We speculate that these structures may contain putative replication forks. Overall, the experiments presented here demonstrate the viability of using fluid fixation for the preparation of DNA molecules for SFM imaging. The combination of largely automatable optically based techniques with the high-resolution SFM imaging presented here will likely produce a high-throughput system for detailed physical mapping of genomic DNA or clones.

Biophys J, July 1998, p. 513-520, Vol. 75, No. 1
© 1998 by the Biophysical Society   0006-3495/98/07/513/08  $2.00


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
T.-F. Chan, C. Ha, A. Phong, D. Cai, E. Wan, L. Leung, P.-Y. Kwok, and M. Xiao
A simple DNA stretching method for fluorescence imaging of single DNA molecules
Nucleic Acids Res., October 18, 2006; 34(17): e113 - e113.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Otobe and T. Ohtani
Behavior of DNA fibers stretched by precise meniscus motion control
Nucleic Acids Res., November 15, 2001; 29(22): e109 - e109.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. Lin, R. Qi, C. Aston, J. Jing, T. S. Anantharaman, B. Mishra, O. White, M. J. Daly, K. W. Minton, J. C. Venter, et al.
Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans
Science, September 3, 1999; 285(5433): 1558 - 1562.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Biophysical Society.