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Biophys J, August 1998, p. 810-824, Vol. 75, No. 2

Voltage and Cosubstrate Dependence of the Na-HCO3 Cotransporter Kinetics in Renal Proximal Tubule Cells

Eitan Gross and Ulrich Hopfer

Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106-4970 USA

The voltage dependence of the kinetics of the sodium bicarbonate cotransporter was studied in proximal tubule cells. This electrogenic cotransporter transports one Na+, three HCO3-, and two negative charges. Cells were grown to confluence on a permeable support, mounted on a Ussing-type chamber, and permeabilized apically to small monovalent ions with amphotericin B. The steady-state, di-nitro-stilbene-di-sulfonate-sensitive current was shown to be sodium and bicarbonate dependent and therefore was taken as flux through the cotransporter. Voltage-current relations were measured as a function of Na+ and HCO3- concentrations between -160 and +160 mV under zero-trans and symmetrical conditions. The kinetics could be described by a Michaelis-Menten behavior with a Hill coefficient of 3 for HCO3- and 1 for Na+. The data were fitted to six-state ordered binding models without restrictions with respect to the rate-limiting step. All ordered models could quantitatively account for the observed current-voltage relationships and the transinhibition by high bicarbonate concentration. The models indicate that 1) the unloaded transporter carries a positive charge; 2) the binding of cytosolic bicarbonate to the transporter "senses" 12% of the electric field in the membrane, whereas its translocation across the membrane "senses" 88% of the field; 3) the binding of Na+ to the cotransporter is voltage independent.

Biophys J, August 1998, p. 810-824, Vol. 75, No. 2
© 1998 by the Biophysical Society   0006-3495/98/08/810/15  $2.00


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