help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tang, C.-Y.
Right arrow Articles by Papazian, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tang, C.-Y.
Right arrow Articles by Papazian, D. M.

Biophys J, September 1998, p. 1263-1270, Vol. 75, No. 3

Shaker and Ether-à-Go-Go K+ Channel Subunits Fail to Coassemble in Xenopus Oocytes

Chih-Yung Tang,* Christine T. Schulteis,# Rhina M. Jiménez,* and Diane M. Papazian*#§

 *Department of Physiology,  #Interdepartmental Program in Neuroscience, and  §Molecular Biology Institute, UCLA School of Medicine, Los Angeles, California 90095-1751 USA

Members of different voltage-gated K+ channel subfamilies usually do not form heteromultimers. However, coassembly between Shaker and ether-à-go-go (eag) subunits, members of two distinct K+ channel subfamilies, was suggested by genetic and functional studies (Zhong and Wu. 1991. Science. 252:1562-1564; Chen, M.-L., T. Hoshi, and C.-F. Wu. 1996. Neuron. 17:535-542). We investigated whether Shaker and eag form heteromultimers in Xenopus laevis oocytes using electrophysiological and biochemical approaches. Coexpression of Shaker and eag subunits produced K+ currents that were virtually identical to the sum of separate Shaker and eag currents, with no change in the kinetics of Shaker inactivation. According to the results of dominant negative and reciprocal coimmunoprecipitation experiments, the Shaker and eag proteins do not interact. We conclude that Shaker and eag do not coassemble to form heteromultimers in Xenopus oocytes.

Biophys J, September 1998, p. 1263-1270, Vol. 75, No. 3
© 1998 by the Biophysical Society   0006-3495/98/09/1263/08  $2.00


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. T. Piros, L. Shen, and X.-Y. Huang
Purification of an EH Domain-binding Protein from Rat Brain That Modulates the Gating of the Rat ether-a-go-go Channel
J. Biol. Chem., November 19, 1999; 274(47): 33677 - 33683.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. T. Schulteis, N. Nagaya, and D. M. Papazian
Subunit Folding and Assembly Steps Are Interspersed during Shaker Potassium Channel Biogenesis
J. Biol. Chem., October 2, 1998; 273(40): 26210 - 26217.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Biophysical Society.