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Biophys J, November 1998, p. 2163-2169, Vol. 75, No. 5

Novel Chelate-Induced Magnetic Alignment of Biological Membranes

R. Scott Prosser, V. B. Volkov, and I. V. Shiyanovskaya

Department of Chemistry, Kent State University, Kent, Ohio 44242 USA

A phospholipid chelate complexed with ytterbium (DMPE-DTPA:Yb3+) is shown to be readily incorporated into a model membrane system, which may then be aligned in a magnetic field such that the average bilayer normal lies along the field. This so-called positively ordered smectic phase, whose lipids consist of less than 1% DMPE-DTPA:Yb3+, is ideally suited to structural studies of membrane proteins by solid-state NMR, low-angle diffraction, and spectroscopic techniques that require oriented samples. The chelate, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine diethylenetriaminepentaacetic acid, which strongly binds the lanthanide ions and serves to orient the membrane in a magnetic field, prevents direct lanthanide-protein interactions and significantly reduces paramagnetic shifts and line broadening. Similar low-spin lanthanide chelates may have applications in field-ordered solution NMR studies of water-soluble proteins and in the design of new magnetically aligned liquid crystalline phases.

Biophys J, November 1998, p. 2163-2169, Vol. 75, No. 5
© 1998 by the Biophysical Society   0006-3495/98/11/2163/07  $2.00


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