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Biophys J, August 1999, p. 888-902, Vol. 77, No. 2

31P NMR First Spectral Moment Study of the Partial Magnetic Orientation of Phospholipid Membranes

Frédéric Picard, Marie-Josée Paquet, Jérôme Levesque, Anne Bélanger, and Michèle Auger

Département de Chimie, Centre de Recherche en Sciences et Ingénierie des Macromolécules, Université Laval, Québec, Québec G1K 7P4, Canada

Structural data can be obtained on proteins inserted in magnetically oriented phospholipid membranes such as bicelles, which are most often made of a mixture of long and short chain phosphatidylcholine. Possible shapes for these magnetically oriented membranes have been postulated in the literature, such as discoidal structures with a thickness of one bilayer and with the short acyl chain phosphatidylcholine on the edges. In the present paper, a geometrical study of these oriented structures is done to determine the validity of this model. The method used is based on the determination of the first spectral moment of solid-state 31P nuclear magnetic resonance spectra. From this first moment, an order parameter is defined that allows a quantitative analysis of partially oriented spectra. The validity of this method is demonstrated in the present study for oriented samples made of DMPC, DMPC:DHPC, DMPC:DHPC:gramicidin A and adriamycin:cardiolipin.

Biophys J, August 1999, p. 888-902, Vol. 77, No. 2
© 1999 by the Biophysical Society   0006-3495/99/08/888/15  $2.00


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