Biophys J, September 1999, p. 1257-1267, Vol. 77, No. 3

The Osmotic Migration of Cells in a Solute Gradient

 ^*Institut Universitaire des Systèmes Thermiques IndustrielsCentre National de la Recherche Scientifique UMR 6595, Université de Provence, 13453 Marseille Cedex 13, France, and  ^#Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109-2121 USA

The effect of a nonuniform solute concentration on the osmotic transport of water through the boundaries of a simple model cell is investigated. A system of two ordinary differential equations is derived for the motion of a single cell in the limit of a fast solute diffusion, and an analytic solution is obtained for one special case. A two-dimensional finite element model has been developed to simulate the more general case (finite diffusion rates, solute gradient induced by a solidification front). It is shown that the cell moves to regions of lower solute concentration due to the uneven flux of water through the cell boundaries. This mechanism has apparently not been discussed previously. The magnitude of this effect is small for red blood cells, the case in which all of the relevant parameters are known. We show, however, that it increases with cell size and membrane permeability, so this effect could be important for larger cells. The finite element model presented should also have other applications in the study of the response of cells to an osmotic stress and for the interaction of cells and solidification fronts. Such investigations are of major relevance for the optimization of cryopreservation processes.

Biophys J, September 1999, p. 1257-1267, Vol. 77, No. 3
© 1999 by the Biophysical Society   0006-3495/99/09/1257/11  $2.00