help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abney, J. R.
Right arrow Articles by Scalettar, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abney, J. R.
Right arrow Articles by Scalettar, B. A.

Biophys J, November 1999, p. 2887-2895, Vol. 77, No. 5

Real-Time Imaging of the Dynamics of Secretory Granules in Growth Cones

James R. Abney,*# C. Daniel Meliza,§ Bryan Cutler,* Mary Kingma,§ Janis E. Lochner,§ and Bethe A. Scalettar*

Departments of  *Physics and  §Chemistry, Lewis and Clark College, Portland, Oregon 97219, and  #Kolisch, Hartwell, Dickinson, McCormack, and Heuser, P.C., Intellectual Property Attorneys, Portland, Oregon 97204 USA

Secretory granules containing a hybrid protein consisting of the regulated secretory protein tissue plasminogen activator and an enhanced form of green fluorescent protein were tracked at high spatial resolution in growth cones of differentiated PC12 cells. Tracking shows that granules, unlike synaptic vesicles, generally are mobile in growth cones. Quantitative analysis of trajectories generated by granules revealed two dominant modes of motion: diffusive and directed. Diffusive motion was observed primarily in central and peripheral parts of growth cones, where most granules diffused two to four orders of magnitude more slowly than comparably sized spheres in dilute solution. Directed motion was observed primarily in proximal parts of growth cones, where a subset of granules underwent rapid, directed motion at average speeds comparable to those observed for granules in neurites. This high-resolution view of the dynamics of secretory granules in growth cones provides insight into granule organization and release at nerve terminals. In particular, the mobility of granules suggests that granules, unlike synaptic vesicles, are not tethered stably to cytoskeletal structures in nerve terminals. Moreover, the slow diffusive nature of this mobility suggests that secretory responses involving centrally distributed granules in growth cones will occur slowly, on a time scale of minutes or longer.

Biophys J, November 1999, p. 2887-2895, Vol. 77, No. 5
© 1999 by the Biophysical Society   0006-3495/99/11/2887/09  $2.00


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
M. L. Chabrillat, C. Wilhelm, C. Wasmeier, E. V. Sviderskaya, D. Louvard, and E. Coudrier
Rab8 Regulates the Actin-based Movement of Melanosomes
Mol. Biol. Cell, April 1, 2005; 16(4): 1640 - 1650.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Silverman, S. Johnson, D. Gurkins, M. Farmer, J. E. Lochner, P. Rosa, and B. A. Scalettar
Mechanisms of Transport and Exocytosis of Dense-Core Granules Containing Tissue Plasminogen Activator in Developing Hippocampal Neurons
J. Neurosci., March 23, 2005; 25(12): 3095 - 3106.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Aschenbrenner, S. N. Naccache, and T. Hasson
Uncoated Endocytic Vesicles Require the Unconventional Myosin, Myo6, for Rapid Transport through Actin Barriers
Mol. Biol. Cell, May 1, 2004; 15(5): 2253 - 2263.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y.-K. Ng, X. Lu, A. Gulacsi, W. Han, M. J. Saxton, and E. S. Levitan
Unexpected Mobility Variation among Individual Secretory Vesicles Produces an Apparent Refractory Neuropeptide Pool
Biophys. J., June 1, 2003; 84(6): 4127 - 4134.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y.-K. Ng, X. Lu, S. C. Watkins, G. C. R. Ellis-Davies, and E. S. Levitan
Nerve Growth Factor-Induced Differentiation Changes the Cellular Organization of Regulated Peptide Release by PC12 Cells
J. Neurosci., May 15, 2002; 22(10): 3890 - 3897.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. A. Scalettar, P. Rosa, E. Taverna, M. Francolini, T. Tsuboi, S. Terakawa, S. Koizumi, J. Roder, and A. Jeromin
Neuronal calcium sensor-1 binds to regulated secretory organelles and functions in basal and stimulated exocytosis in PC12 cells
J. Cell Sci., January 6, 2002; 115(11): 2399 - 2412.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M.-N. Cordonnier, D. Dauzonne, D. Louvard, and E. Coudrier
Actin Filaments and Myosin I Alpha Cooperate with Microtubules for the Movement of Lysosomes
Mol. Biol. Cell, December 1, 2001; 12(12): 4013 - 4029.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the Biophysical Society.