| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophys J, December 1999, p. 3034-3042, Vol. 77, No. 6
1H
Department of Physiology, Loyola University Medical Center, Maywood, Illinois 60153 USA
Nickel has been proposed to be a selective blocker of
low-voltage-activated, T-type calcium channels. However, studies on cloned high-voltage-activated Ca2+ channels indicated that
some subtypes, such as 
1E, are also blocked by low micromolar
concentrations of NiCl2. There are considerable differences
in the sensitivity to Ni2+ among native T-type currents,
leading to the hypothesis that there may be more than one T-type
channel. We confirmed part of this hypothesis by cloning three novel
Ca2+ channels, 1G, H, and I, whose currents are nearly
identical to the biophysical properties of native T-type channels. In
this study we examined the nickel block of these cloned T-type channels expressed in both Xenopus oocytes and HEK-293 cells (10 mM Ba2+). Only 1H currents were sensitive to low
micromolar concentrations (IC50 = 13 µM). Much
higher concentrations were required to half-block 1I (216 µM) and
1G currents (250 µM). Nickel block varied with the test potential,
with less block at potentials above 
30 mV. Outward currents through
the T channels were blocked even less. We show that depolarizations can
unblock the channel and that this can occur in the absence of
permeating ions. We conclude that Ni2+ is only a selective
blocker of 1H currents and that the concentrations required to block
1G and 1I will also affect high-voltage-activated calcium currents.
Biophys J, December 1999, p. 3034-3042, Vol. 77, No. 6
© 1999 by the Biophysical Society 0006-3495/99/12/3034/09 $2.00
This article has been cited by other articles:
 |
|
 |
|
  C. A. Obejero-Paz, I. P. Gray, and S. W. Jones Ni2+ Block of CaV3.1 ({alpha}1G) T-type Calcium Channels J. Gen. Physiol., August 1, 2008; 132(2): 239 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sun, D. Varela, D. Chartier, P. C. Ruben, S. Nattel, G. W. Zamponi, and N. Leblanc Differential Interactions of Na+ Channel Toxins with T-type Ca2+ Channels J. Gen. Physiol., June 30, 2008; 132(1): 101 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Jagodic, S. Pathirathna, P. M. Joksovic, W. Lee, M. T. Nelson, A. K. Naik, P. Su, V. Jevtovic-Todorovic, and S. M. Todorovic Upregulation of the T-Type Calcium Current in Small Rat Sensory Neurons After Chronic Constrictive Injury of the Sciatic Nerve J Neurophysiol, June 1, 2008; 99(6): 3151 - 3156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Gackiere, G. Bidaux, P. Delcourt, F. Van Coppenolle, M. Katsogiannou, E. Dewailly, A. Bavencoffe, M. T. Van Chuoi-Mariot, B. Mauroy, N. Prevarskaya, et al. CaV3.2 T-type Calcium Channels Are Involved in Calcium-dependent Secretion of Neuroendocrine Prostate Cancer Cells J. Biol. Chem., April 11, 2008; 283(15): 10162 - 10173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-H. Lai, Y.-F. Lin, F.-C. Wu, and Y.-H. Tsai Follicle-Stimulating Hormone-Induced G{alpha}h/Phospholipase C-{delta}1 Signaling Mediating a Noncapacitative Ca2+ Influx through T-Type Ca2+ Channels in Rat Sertoli Cells Endocrinology, March 1, 2008; 149(3): 1031 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chen and Y. Yaari Spike Ca2+ influx upmodulates the spike afterdepolarization and bursting via intracellular inhibition of KV7/M channels J. Physiol., March 1, 2008; 586(5): 1351 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Carabelli, A. Marcantoni, V. Comunanza, A. de Luca, J. Diaz, R. Borges, and E. Carbone Chronic hypoxia up-regulates {alpha}1H T-type channels and low-threshold catecholamine secretion in rat chromaffin cells J. Physiol., October 1, 2007; 584(1): 149 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Hammack, I. Mania, and D. G. Rainnie Differential Expression of Intrinsic Membrane Currents in Defined Cell Types of the Anterolateral Bed Nucleus of the Stria Terminalis J Neurophysiol, August 1, 2007; 98(2): 638 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Blanks, Z.-H. Zhao, A. Shmygol, G. Bru-Mercier, S. Astle, and S. Thornton Characterization of the molecular and electrophysiological properties of the T-type calcium channel in human myometrium J. Physiol., June 15, 2007; 581(3): 915 - 926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yaari, C. Yue, and H. Su Recruitment of apical dendritic T-type Ca2+ channels by backpropagating spikes underlies de novo intrinsic bursting in hippocampal epileptogenesis J. Physiol., April 15, 2007; 580(2): 435 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Jagodic, S. Pathirathna, M. T. Nelson, S. Mancuso, P. M. Joksovic, E. R. Rosenberg, D. A. Bayliss, V. Jevtovic-Todorovic, and S. M. Todorovic Cell-Specific Alterations of T-Type Calcium Current in Painful Diabetic Neuropathy Enhance Excitability of Sensory Neurons J. Neurosci., March 21, 2007; 27(12): 3305 - 3316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chen, R. M. Wilson, H. Kubo, R. M. Berretta, D. M. Harris, X. Zhang, N. Jaleel, S. M. MacDonnell, C. Bearzi, J. Tillmanns, et al. Adolescent Feline Heart Contains a Population of Small, Proliferative Ventricular Myocytes With Immature Physiological Properties Circ. Res., March 2, 2007; 100(4): 536 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Rosati, W. Dun, M. Hirose, P. A. Boyden, and D. McKinnon Molecular basis of the T- and L-type Ca2+ currents in canine Purkinje fibres J. Physiol., March 1, 2007; 579(2): 465 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Traboulsie, J. Chemin, M. Chevalier, J.-F. Quignard, J. Nargeot, and P. Lory Subunit-specific modulation of T-type calcium channels by zinc J. Physiol., January 1, 2007; 578(1): 159 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Michels, F. Er, M. Eicks, S. Herzig, and U. C. Hoppe Long-Term and Immediate Effect of Testosterone on Single T-Type Calcium Channel in Neonatal Rat Cardiomyocytes Endocrinology, November 1, 2006; 147(11): 5160 - 5169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Ivanov and R. L. Calabrese Graded Inhibitory Synaptic Transmission Between Leech Interneurons: Assessing the Roles of Two Kinetically Distinct Low-Threshold Ca Currents J Neurophysiol, July 1, 2006; 96(1): 218 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Mangoni, A. Traboulsie, A.-L. Leoni, B. Couette, L. Marger, K. Le Quang, E. Kupfer, A. Cohen-Solal, J. Vilar, H.-S. Shin, et al. Bradycardia and Slowing of the Atrioventricular Conduction in Mice Lacking CaV3.1/{alpha}1G T-Type Calcium Channels Circ. Res., June 9, 2006; 98(11): 1422 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tai, J. B. Kuzmiski, and B. A. MacVicar Muscarinic enhancement of R-type calcium currents in hippocampal CA1 pyramidal neurons. J. Neurosci., June 7, 2006; 26(23): 6249 - 6258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Giancippoli, M. Novara, A. de Luca, P. Baldelli, A. Marcantoni, E. Carbone, and V. Carabelli Low-Threshold Exocytosis Induced by cAMP-Recruited CaV3.2 ({alpha}1H) Channels in Rat Chromaffin Cells Biophys. J., March 1, 2006; 90(5): 1830 - 1841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Bergh, Y. Shao, E. Puente, R. L. Duncan, and M. C. Farach-Carson Osteoblast Ca2+ permeability and voltage-sensitive Ca2+ channel expression is temporally regulated by 1,25-dihydroxyvitamin D3 Am J Physiol Cell Physiol, March 1, 2006; 290(3): C822 - C831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Moosmang, N. Haider, B. Bruderl, A. Welling, and F. Hofmann Antihypertensive Effects of the Putative T-Type Calcium Channel Antagonist Mibefradil Are Mediated by the L-Type Calcium Channel Cav1.2 Circ. Res., January 6, 2006; 98(1): 105 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Autret, I. Mechaly, F. Scamps, J. Valmier, P. Lory, and G. Desmadryl The involvement of Cav3.2/{alpha}1H T-type calcium channels in excitability of mouse embryonic primary vestibular neurones J. Physiol., August 15, 2005; 567(1): 67 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Kim, J.-Y. Park, H.-W. Kang, E.-J. Lee, H. Bang, and J.-H. Lee Zinc Activates TREK-2 Potassium Channel Activity J. Pharmacol. Exp. Ther., August 1, 2005; 314(2): 618 - 625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Laleve, M. C. Rebsamen, S. Barrere-Lemaire, E. Perrier, J. Nargeot, J.-P. Benitah, and M. F. Rossier Aldosterone increases T-type calcium channel expression and in vitro beating frequency in neonatal rat cardiomyocytes Cardiovasc Res, August 1, 2005; 67(2): 216 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M Joksovic, D. A Bayliss, and S. M Todorovic Different kinetic properties of two T-type Ca2+ currents of rat reticular thalamic neurones and their modulation by enflurane J. Physiol., July 1, 2005; 566(1): 125 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Metz, T. Jarsky, M. Martina, and N. Spruston R-Type Calcium Channels Contribute to Afterdepolarization and Bursting in Hippocampal CA1 Pyramidal Neurons J. Neurosci., June 15, 2005; 25(24): 5763 - 5773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Shtonda and L. Avery CCA-1, EGL-19 and EXP-2 currents shape action potentials in the Caenorhabditis elegans pharynx J. Exp. Biol., June 1, 2005; 208(11): 2177 - 2190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Prescott and Y. De Koninck Integration Time in a Subset of Spinal Lamina I Neurons Is Lengthened by Sodium and Calcium Currents Acting Synergistically to Prolong Subthreshold Depolarization J. Neurosci., May 11, 2005; 25(19): 4743 - 4754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Spanswick and L. P. Renaud Angiotensin II Induces Calcium-Dependent Rhythmic Activity in a Subpopulation of Rat Hypothalamic Median Preoptic Nucleus Neurons J Neurophysiol, April 1, 2005; 93(4): 1970 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Larsen, C.-C. Chen, and P. M. Best Disruption of growth hormone secretion alters Ca2+ current density and expression of Ca2+ channel and insulin-like growth factor genes in rat atria Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H829 - H838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. I. Brueggemann, B. L. Martin, J. Barakat, K. L. Byron, and L. L. Cribbs Low voltage-activated calcium channels in vascular smooth muscle: T-type channels and AVP-stimulated calcium spiking Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H923 - H935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Isope and T. H. Murphy Low threshold calcium currents in rat cerebellar Purkinje cell dendritic spines are mediated by T-type calcium channels J. Physiol., January 1, 2005; 562(1): 257 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Cui, T. Okamoto, and H. Morikawa Spontaneous Opening of T-Type Ca2+ Channels Contributes to the Irregular Firing of Dopamine Neurons in Neonatal Rats J. Neurosci., December 8, 2004; 24(49): 11079 - 11087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Carbone Ion Trafficking through T-type Ca2+ Channels: A Way to Look at Channel Gating Position J. Gen. Physiol., December 1, 2004; 124(6): 619 - 622. [Full Text] [PDF]
|
|
|
|
|
|
C. A. Obejero-Paz, I. P. Gray, and S. W. Jones Y3+ Block Demonstrates an Intracellular Activation Gate for the {alpha}1G T-type Ca2+ Channel J. Gen. Physiol., November 29, 2004; 124(6): 631 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-S. Dubreuil, H. Boukhaddaoui, G. Desmadryl, C. Martinez-Salgado, R. Moshourab, G. R. Lewin, P. Carroll, J. Valmier, and F. Scamps Role of T-Type Calcium Current in Identified D-Hair Mechanoreceptor Neurons Studied In Vitro J. Neurosci., September 29, 2004; 24(39): 8480 - 8484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Agoston, L. Kunz, A. Krieger, and A. Mayerhofer Two Types of Calcium Channels in Human Ovarian Endocrine Cells: Involvement in Steroidogenesis J. Clin. Endocrinol. Metab., September 1, 2004; 89(9): 4503 - 4512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. N. Liu, T. Kurotani, M. Ren, K. Yamada, Y. Yoshimura, and Y. Komatsu Presynaptic Activity and Ca2+ Entry Are Required for the Maintenance of NMDA Receptor-Independent LTP at Visual Cortical Excitatory Synapses J Neurophysiol, August 1, 2004; 92(2): 1077 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Novara, P. Baldelli, D. Cavallari, V. Carabelli, A. Giancippoli, and E. Carbone Exposure to cAMP and {beta}-adrenergic stimulation recruits CaV3 T-type channels in rat chromaffin cells through Epac cAMP-receptor proteins J. Physiol., July 15, 2004; 558(2): 433 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Salthun-Lassalle, E. C. Hirsch, J. Wolfart, M. Ruberg, and P. P. Michel Rescue of Mesencephalic Dopaminergic Neurons in Culture by Low-Level Stimulation of Voltage-Gated Sodium Channels J. Neurosci., June 30, 2004; 24(26): 5922 - 5930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Jensen, U. G. Friis, P. B. Hansen, D. Andreasen, T. Uhrenholt, J. Schjerning, and O. Skott Voltage-dependent calcium channels in the renal microcirculation Nephrol. Dial. Transplant., June 1, 2004; 19(6): 1368 - 1373. [Full Text] [PDF]
|
|
|
|
|
|
N. Niwa, K. Yasui, T. Opthof, H. Takemura, A. Shimizu, M. Horiba, J.-K. Lee, H. Honjo, K. Kamiya, and I. Kodama Cav3.2 subunit underlies the functional T-type Ca2+ channel in murine hearts during the embryonic period Am J Physiol Heart Circ Physiol, June 1, 2004; 286(6): H2257 - H2263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Bradley, U. A. Anderson, S. M. Woolsey, K. D. Thornbury, N. G. McHale, and M. A. Hollywood Characterization of T-type calcium current and its contribution to electrical activity in rabbit urethra Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1078 - C1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ferron, V. Capuano, Y. Ruchon, E. Deroubaix, A. Coulombe, and J.-F. Renaud Angiotensin II Signaling Pathways Mediate Expression of Cardiac T-Type Calcium Channels Circ. Res., December 12, 2003; 93(12): 1241 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Lee, Y. Hayashida, and A. T. Ishida Availability of Low-Threshold Ca2+ Current in Retinal Ganglion Cells J Neurophysiol, December 1, 2003; 90(6): 3888 - 3901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Zhang, L. Shang, C. Hartzell, M. Narlow, L. Cribbs, and S. C. Dudley Jr. Characterization and regulation of T-type Ca2+ channels in embryonic stem cell-derived cardiomyocytes Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2770 - H2779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pinato and J. Midtgaard Regulation of Granule Cell Excitability by a Low-Threshold Calcium Spike in Turtle Olfactory Bulb J Neurophysiol, November 1, 2003; 90(5): 3341 - 3351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wu, J. Haynes Jr, J. T. Taylor, B. O. Obiako, J. R. Stubbs, M. Li, and T. Stevens Cav3.1 ({alpha}1G) T-Type Ca2+ Channels Mediate Vaso-Occlusion of Sickled Erythrocytes in Lung Microcirculation Circ. Res., August 22, 2003; 93(4): 346 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Del Toro, K. L. Levitsky, J. Lopez-Barneo, and M. D. Chiara Induction of T-type Calcium Channel Gene Expression by Chronic Hypoxia J. Biol. Chem., June 13, 2003; 278(25): 22316 - 22324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Talavera, A. Janssens, N. Klugbauer, G. Droogmans, and B. Nilius Extracellular Ca2+ Modulates the Effects of Protons on Gating and Conduction Properties of the T-type Ca2+ Channel {alpha}1G (CaV3.1) J. Gen. Physiol., May 27, 2003; 121(6): 511 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Cloues and W. A. Sather Afterhyperpolarization Regulates Firing Rate in Neurons of the Suprachiasmatic Nucleus J. Neurosci., March 1, 2003; 23(5): 1593 - 1604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chemin, J. Nargeot, and P. Lory Neuronal T-type alpha 1H Calcium Channels Induce Neuritogenesis and Expression of High-Voltage-Activated Calcium Channels in the NG108-15 Cell Line J. Neurosci., August 15, 2002; 22(16): 6856 - 6862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Lee, E.-G. Kim, B.-G. Park, K.-H. Kim, S.-K. Cha, I. D. Kong, J.-W. Lee, and S.-W. Jeong Identification of T-Type alpha 1H Ca2+ Channels (Cav3.2) in Major Pelvic Ganglion Neurons J Neurophysiol, June 1, 2002; 87(6): 2844 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Su, D. Sochivko, A. Becker, J. Chen, Y. Jiang, Y. Yaari, and H. Beck Upregulation of a T-Type Ca2+ Channel Causes a Long-Lasting Modification of Neuronal Firing Mode after Status Epilepticus J. Neurosci., May 1, 2002; 22(9): 3645 - 3655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mariot, K. Vanoverberghe, N. Lalevee, M. F. Rossier, and N. Prevarskaya Overexpression of an alpha 1H (Cav3.2) T-type Calcium Channel during Neuroendocrine Differentiation of Human Prostate Cancer Cells J. Biol. Chem., March 22, 2002; 277(13): 10824 - 10833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Gomora, A. N. Daud, M. Weiergraber, and E. Perez-Reyes Block of Cloned Human T-Type Calcium Channels by Succinimide Antiepileptic Drugs Mol. Pharmacol., November 1, 2001; 60(5): 1121 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Lesouhaitier, A. Chiappe, and M. F. Rossier Aldosterone Increases T-Type Calcium Currents in Human Adrenocarcinoma (H295R) Cells by Inducing Channel Expression Endocrinology, October 1, 2001; 142(10): 4320 - 4330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Protas, D. DiFrancesco, and R. B. Robinson L-type but not T-type calcium current changes during postnatal development in rabbit sinoatrial node Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1252 - H1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-y. Jung, N. P. Staff, and N. Spruston Action Potential Bursting in Subicular Pyramidal Neurons Is Driven by a Calcium Tail Current J. Neurosci., May 15, 2001; 21(10): 3312 - 3321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Brevi, M. de Curtis, and J. Magistretti Pharmacological and Biophysical Characterization of Voltage-Gated Calcium Currents in the Endopiriform Nucleus of the Guinea Pig J Neurophysiol, May 1, 2001; 85(5): 2076 - 2087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Cribbs, B. L. Martin, E. A. Schroder, B. B. Keller, B. P. Delisle, and J. Satin Identification of the T-Type Calcium Channel (CaV3.1d) in Developing Mouse Heart Circ. Res., March 2, 2001; 88(4): 403 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Schrier, H. Wang, E. M. Talley, E. Perez-Reyes, and P. Q. Barrett {alpha}1H T-type Ca2+ channel is the predominant subtype expressed in bovine and rat zona glomerulosa Am J Physiol Cell Physiol, February 1, 2001; 280(2): C265 - C272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
