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Biophys J, December 1999, p. 3227-3233, Vol. 77, No. 6
*Department of Chemistry & Biochemistry, University of California, Santa Cruz, CA 95064; #Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA 02138; and §Biotechnology Division, National Institute of Standards & Technology, Gaithersburg, MD 20899
Single molecules of DNA or RNA can be detected as they
are driven through an 
-hemolysin channel by an applied electric
field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential, single-file order because
the limiting diameter of the pore can accommodate only one strand of
DNA or RNA at a time. Here we demonstrate that this nanopore behaves as
a detector that can rapidly discriminate between pyrimidine and purine
segments along an RNA molecule. Nanopore detection and characterization
of single molecules represent a new method for directly reading
information encoded in linear polymers, and are critical first steps
toward direct sequencing of individual DNA and RNA molecules.
Biophys J, December 1999, p. 3227-3233, Vol. 77, No. 6
© 1999 by the Biophysical Society 0006-3495/99/12/3227/07 $2.00
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