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Biophys J, January 2000, p. 513-519, Vol. 78, No. 1

Computerized Analysis of Chemotaxis at Different Stages of Bacterial Growth

John F. Staropoli* and Uri Alon*dagger

Departments of  *Molecular Biology and  dagger Physics, Princeton University, Princeton, NJ 08544

Chemotactic behavior as a function of growth stage in an Escherichia coli strain commonly used for chemotaxis studies was characterized using computerized image analysis. The response and adaptation to saturating, step-like additions of the attractant L-aspartate were measured. Steady-state average tumbling frequency and adaptation time increased nearly twofold during logarithmic phase. In contrast, precision of adaptation, P, defined as the ratio between steady-state tumbling frequencies in the presence and absence of attractant, appeared to be constant throughout growth (P = 1.0 ± 0.2). The variation of tumble duration over growth was consistent with a hydrodynamic mechanism for tumble termination.

Biophys J, January 2000, p. 513-519, Vol. 78, No. 1
© 2000 by the Biophysical Society   0006-3495/00/01/513/07  $2.00




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