| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophys J, March 2000, p. 1349-1358, Vol. 78, No. 3
and
*Wadsworth Center for Laboratories and Research, New York State
Department of Health, Albany, New York 12201-0509 USA;
School of Public Health State University of New York at
Albany, Albany, New York 12201-0509 USA; and Institute
of General Pathology, University of Ferrara, 44100 Ferrara, Italy
We have localized a region contained within the sequence
of amino acid residues 4425-4621 on the three-dimensional structure of
the skeletal muscle ryanodine receptor (RyR). Mouse monoclonal antibodies raised against a peptide comprising these residues have been
complexed with ryanodine receptors and imaged in the frozen-hydrated
state by cryoelectron microscopy. These images, along with images of
antibody-free ryanodine receptor, were used to compute two-dimensional
averaged images and three-dimensional reconstructions. Two-dimensional
averages of immunocomplexes in which the ryanodine receptor was in the
fourfold symmetrical orientation disclosed four symmetrical regions of
density located on the edges of the receptor's cytoplasmic assembly
that were absent from control averages of receptor without added
antibody. Three-dimensional reconstructions revealed the
antibody-binding sites to be on the so-called handle domains of the
ryanodine receptor's cytoplasmic assembly, near their junction with
the transmembrane assembly. This study is the first to demonstrate
epitope mapping on the three-dimensional structure of the ryanodine receptor.
Biophys J, March 2000, p. 1349-1358, Vol. 78, No. 3
© 2000 by the Biophysical Society 0006-3495/00/03/1349/10 $2.00
This article has been cited by other articles:
 |
|
 |
|
  A. J. Tanskanen, J. L. Greenstein, A. Chen, S. X. Sun, and R. L. Winslow Protein Geometry and Placement in the Cardiac Dyad Influence Macroscopic Properties of Calcium-Induced Calcium Release Biophys. J., May 15, 2007; 92(10): 3379 - 3396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Romi, G. O. Skeie, N. E. Gilhus, and J. A. Aarli Striational Antibodies in Myasthenia Gravis: Reactivity and Possible Clinical Significance Arch Neurol, March 1, 2005; 62(3): 442 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ducreux, F. Zorzato, C. Muller, C. Sewry, F. Muntoni, R. Quinlivan, G. Restagno, T. Girard, and S. Treves Effect of Ryanodine Receptor Mutations on Interleukin-6 Release and Intracellular Calcium Homeostasis in Human Myotubes from Malignant Hyperthermia-susceptible Individuals and Patients Affected by Central Core Disease J. Biol. Chem., October 15, 2004; 279(42): 43838 - 43846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, J. Zhang, P. Li, S. R. W. Chen, and T. Wagenknecht Three-dimensional Reconstruction of the Recombinant Type 2 Ryanodine Receptor and Localization of Its Divergent Region 1 J. Biol. Chem., November 22, 2002; 277(48): 46712 - 46719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Girard, D. Cavagna, E. Padovan, G. Spagnoli, A. Urwyler, F. Zorzato, and S. Treves B-lymphocytes from Malignant Hyperthermia-susceptible Patients Have an Increased Sensitivity to Skeletal Muscle Ryanodine Receptor Activators J. Biol. Chem., December 14, 2001; 276(51): 48077 - 48082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Liu, J. Zhang, M. R. Sharma, P. Li, S. R. W. Chen, and T. Wagenknecht Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus PNAS, May 22, 2001; 98(11): 6104 - 6109. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
