help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Noireaux, V.
Right arrow Articles by Sykes, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Noireaux, V.
Right arrow Articles by Sykes, C.

Biophys J, March 2000, p. 1643-1654, Vol. 78, No. 3

Growing an Actin Gel on Spherical Surfaces

V. Noireaux,* R. M. Golsteyn,dagger E. Friederich,dagger J. Prost,* C. Antony,dagger D. Louvard,dagger and C. Sykes*

 *Laboratoire Physico-Chimie "Curie," Unité Mixte de Recherche CNRS/Institut Curie (UMR 168), 75231 Paris Cedex 05, and  dagger Laboratoire Compartimentation et Dynamique Cellulaire, Unité Mixte de Recherche CNRS/Institut Curie (UMR 144), 75248 Paris Cedex 05, France

Inspired by the motility of the bacteria Listeria monocytogenes, we have experimentally studied the growth of an actin gel around spherical beads grafted with ActA, a protein known to be the promoter of bacteria movement. On ActA-grafted beads F-actin is formed in a spherical manner, whereas on the bacteria a "comet-like" tail of F-actin is produced. We show experimentally that the stationary thickness of the gel depends on the radius of the beads. Moreover, the actin gel is not formed if the ActA surface density is too low. To interpret our results, we propose a theoretical model to explain how the mechanical stress (due to spherical geometry) limits the growth of the actin gel. Our model also takes into account treadmilling of actin. We deduce from our work that the force exerted by the actin gel on the bacteria is of the order of 10 pN. Finally, we estimate from our theoretical model possible conditions for developing actin comet tails.

Biophys J, March 2000, p. 1643-1654, Vol. 78, No. 3
© 2000 by the Biophysical Society   0006-3495/00/03/1643/12  $2.00


This article has been cited by other articles:


Home page
 Biophys. JHome page
K.-C. Lee and A. J. Liu
New Proposed Mechanism of Actin-Polymerization-Driven Motility
Biophys. J., November 15, 2008; 95(10): 4529 - 4539.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. Paluch, J. van der Gucht, and C. Sykes
Cracking up: symmetry breaking in cellular systems
J. Cell Biol., December 4, 2006; 175(5): 687 - 692.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
E. Paluch, J. v. d. Gucht, J.-F. Joanny, and C. Sykes
Deformations in Actin Comets from Rocketing Beads
Biophys. J., October 15, 2006; 91(8): 3113 - 3122.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
R. B. Dickinson and D. L. Purich
Diffusion Rate Limitations in Actin-Based Propulsion of Hard and Deformable Particles
Biophys. J., August 15, 2006; 91(4): 1548 - 1563.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. M. Rafelski and J. A. Theriot
Bacterial Shape and ActA Distribution Affect Initiation of Listeria monocytogenes Actin-Based Motility
Biophys. J., September 1, 2005; 89(3): 2146 - 2158.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
E. Paluch, M. Piel, J. Prost, M. Bornens, and C. Sykes
Cortical Actomyosin Breakage Triggers Shape Oscillations in Cells and Cell Fragments
Biophys. J., July 1, 2005; 89(1): 724 - 733.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
R. B. Dickinson, L. Caro, and D. L. Purich
Force Generation by Cytoskeletal Filament End-Tracking Proteins
Biophys. J., October 1, 2004; 87(4): 2838 - 2854.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. A. Cameron, J. R. Robbins, M. J. Footer, and J. A. Theriot
Biophysical Parameters Influence Actin-based Movement, Trajectory, and Initiation in a Cell-free System
Mol. Biol. Cell, May 1, 2004; 15(5): 2312 - 2323.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Marcy, J. Prost, M.-F. Carlier, and C. Sykes
Forces generated during actin-based propulsion: A direct measurement by micromanipulation
PNAS, April 20, 2004; 101(16): 5992 - 5997.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Mogilner and G. Oster
Force Generation by Actin Polymerization II: The Elastic Ratchet and Tethered Filaments
Biophys. J., March 1, 2003; 84(3): 1591 - 1605.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Wiesner, E. Helfer, D. Didry, G. Ducouret, F. Lafuma, M.-F. Carlier, and D. Pantaloni
A biomimetic motility assay provides insight into the mechanism of actin-based motility
J. Cell Biol., February 3, 2003; 160(3): 387 - 398.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
L. Mahadevan and P. Matsudaira
Motility Powered by Supramolecular Springs and Ratchets
Science, April 7, 2000; 288(5463): 95 - 99.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Biophysical Society.