help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berdiev, B. K.
Right arrow Articles by Ismailov, I. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berdiev, B. K.
Right arrow Articles by Ismailov, I. I.

Biophys J, April 2000, p. 1881-1894, Vol. 78, No. 4

Gating of Amiloride-Sensitive Na+ Channels: Subunit-Subunit Interactions and Inhibition by the Cystic Fibrosis Transmembrane Conductance Regulator

Bakhrom K. Berdiev,* Vadim Gh. Shlyonsky,dagger Katherine H. Karlson,Dagger Bruce A. Stanton,Dagger and Iskander I. Ismailov*

 *Department of Physiology and Biophysics and Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294-0005 USA;  dagger Institute of Physiology and Biophysics, Uzbek Academy of Sciences, Tashkent 700095, Uzbekistan; and  Dagger Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire 03755 USA

In search of the structural basis for gating of amiloride-sensitive Na+ channels, kinetic properties of single homo and heterooligomeric ENaCs formed by the subunits with individual truncated cytoplasmic domains were studied in a cell-free planar lipid bilayer reconstitution system. Our results identify the N-terminus of the alpha -subunit as a major determinant of kinetic behavior of both homooligomeric and heterooligomeric ENaCs, although the carboxy-terminal domains of beta - and gamma -ENaC subunits play important role(s) in modulation of the kinetics of heterooligomeric channels. We also found that the cystic fibrosis transmembrane conductance regulator (CFTR) inhibits amiloride-sensitive channels, at least in part, by modulating their gating. Comparison of these data suggests that the modulatory effects of the beta - and gamma -ENaC subunits, and of the CFTR, may involve the same, or closely related, mechanism(s); namely, "locking" the heterooligomeric channels in their closed state. These mechanisms, however, do not completely override the gating mechanism of the alpha -channel.

Biophys J, April 2000, p. 1881-1894, Vol. 78, No. 4
© 2000 by the Biophysical Society   0006-3495/00/04/1881/14  $2.00


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
T. Bachhuber, J. Konig, T. Voelcker, B. Murle, R. Schreiber, and K. Kunzelmann
Cl- Interference with the Epithelial Na+ Channel ENaC
J. Biol. Chem., September 9, 2005; 280(36): 31587 - 31594.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. K. Berdiev, B. Jovov, W. C. Tucker, A. P. Naren, C. M. Fuller, E. R. Chapman, and D. J. Benos
ENaC subunit-subunit interactions and inhibition by syntaxin 1A
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1100 - F1106.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Sheng, C. J. Perry, and T. R. Kleyman
External Nickel Inhibits Epithelial Sodium Channel by Binding to Histidine Residues within the Extracellular Domains of alpha and gamma Subunits and Reducing Channel Open Probability
J. Biol. Chem., December 13, 2002; 277(51): 50098 - 50111.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Gao, J. R. Broughman, T. Iwamoto, J. M. Tomich, C. J. Venglarik, and H. J. Forman
Synthetic chloride channel restores glutathione secretion in cystic fibrosis airway epithelia
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L24 - L30.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-L. Ji, M. L. Chalfant, B. Jovov, J. P. Lockhart, S. B. Parker, C. M. Fuller, B. A. Stanton, and D. J. Benos
The Cytosolic Termini of the beta - and gamma -ENaC Subunits Are Involved in the Functional Interactions between Cystic Fibrosis Transmembrane Conductance Regulator and Epithelial Sodium Channel
J. Biol. Chem., September 1, 2000; 275(36): 27947 - 27956.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Malik, L. Schlanger, O. Al-Khalili, H.-F. Bao, G. Yue, S. R. Price, W. E. Mitch, and D. C. Eaton
ENaC Degradation in A6 Cells by the Ubiquitin-Proteosome Proteolytic Pathway
J. Biol. Chem., April 13, 2001; 276(16): 12903 - 12910.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Biophysical Society.