| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophys J, July 2000, p. 321-327, Vol. 79, No. 1
*Department of Mechanical Engineering and Materials Science, Duke
University, Durham, North Carolina 27708-0300 USA;
Department of Pathology, University of British Columbia,
Vancouver, British Columbia V6T 1W5, Canada; and
Department of Physics, University of British Columbia,
Vancouver, British Columbia V6T 1Z1, Canada
Micropipette aspiration was used to test mechanical
strength and water permeability of giant-fluid bilayer vesicles
composed of polyunsaturated phosphatidylcholine PC lipids. Eight
synthetic-diacyl PCs were chosen with 18 carbon chains and degrees of
unsaturation that ranged from one double bond (C18:0/1, C18:1/0) to six
double bonds per PC molecule (diC18:3). Produced by increasing pipette pressurization, membrane tensions for lysis of single vesicles at
21°C ranged from ~9 to 10 mN/m for mono- and dimono-unsaturated PCs
(18:0/1, 18:1/0, and diC18:1) but dropped abruptly to ~5 mN/m when
one or both PC chains contained two cis-double bonds
(C18:0/2 and diC18:2) and even lower ~3 mN/m for diC18:3. Driven by
osmotic filtration following transfer of individual vesicles to a
hypertonic environment, the apparent coefficient for water permeability
at 21°C varied modestly in a range from ~30 to 40 µm/s for mono- and dimono-unsaturated PCs. However, with two or more
cis-double bonds in a chain, the apparent permeability
rose to ~50 µm/s for C18:0/2, then strikingly to ~90 µm/s for
diC18:2 and ~150 µm/s for diC18:3. The measurements of water
permeability were found to scale exponentially with the reduced
temperatures reported for these lipids in the literature. The
correlation supports the concept that increase in free volume acquired
in thermal expansion above the main gel-liquid crystal transition of a
bilayer is a major factor in water transport. Taken together, the
prominent changes in lysis tension and water permeability indicate that major changes occur in chain packing and cohesive interactions when two
or more cis-double bonds alternate with saturated bonds along a chain.
Biophys J, July 2000, p. 321-327, Vol. 79, No. 1
© 2000 by the Biophysical Society 0006-3495/00/07/321/07 $2.00
This article has been cited by other articles:
 |
|
 |
|
  D. H. Kim and J. A. Frangos Effects of Amyloid {beta}-Peptides on the Lysis Tension of Lipid Bilayer Vesicles Containing Oxysterols Biophys. J., July 15, 2008; 95(2): 620 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Rawicz, B. A. Smith, T. J. McIntosh, S. A. Simon, and E. Evans Elasticity, Strength, and Water Permeability of Bilayers that Contain Raft Microdomain-Forming Lipids Biophys. J., June 15, 2008; 94(12): 4725 - 4736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Mathai, S. Tristram-Nagle, J. F. Nagle, and M. L. Zeidel Structural Determinants of Water Permeability through the Lipid Membrane J. Gen. Physiol., December 31, 2007; 131(1): 69 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Nagle, J. C. Mathai, M. L. Zeidel, and S. Tristram-Nagle Theory of Passive Permeability through Lipid Bilayers J. Gen. Physiol., December 31, 2007; 131(1): 77 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wong-ekkabut, Z. Xu, W. Triampo, I-M. Tang, D. P. Tieleman, and L. Monticelli Effect of Lipid Peroxidation on the Properties of Lipid Bilayers: A Molecular Dynamics Study Biophys. J., December 15, 2007; 93(12): 4225 - 4236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wagner and G. Brezesinski Phospholipase D Activity Is Regulated by Product Segregation and the Structure Formation of Phosphatidic Acid within Model Membranes Biophys. J., October 1, 2007; 93(7): 2373 - 2383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Leontiadou, A. E. Mark, and S.-J. Marrink Ion Transport across Transmembrane Pores Biophys. J., June 15, 2007; 92(12): 4209 - 4215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-A. Boucher, B. Joos, M. J. Zuckermann, and L. Fournier Pore Formation in a Lipid Bilayer under a Tension Ramp: Modeling the Distribution of Rupture Tensions Biophys. J., June 15, 2007; 92(12): 4344 - 4355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Meckel, L. Gall, S. Semrau, U. Homann, and G. Thiel Guard Cells Elongate: Relationship of Volume and Surface Area during Stomatal Movement Biophys. J., February 1, 2007; 92(3): 1072 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Dickinson and D. L. Purich Diffusion Rate Limitations in Actin-Based Propulsion of Hard and Deformable Particles Biophys. J., August 15, 2006; 91(4): 1548 - 1563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Jin, K. Prasad, P. D. Smith, E. M. Lafer, and R. Nossal Measuring the Elasticity of Clathrin-Coated Vesicles via Atomic Force Microscopy Biophys. J., May 1, 2006; 90(9): 3333 - 3344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garcia-Manyes, G. Oncins, and F. Sanz Effect of Temperature on the Nanomechanics of Lipid Bilayers Studied by Force Spectroscopy Biophys. J., December 1, 2005; 89(6): 4261 - 4274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. P. Sugar, N. K. Mizuno, and H. L. Brockman Peripheral Protein Adsorption to Lipid-Water Interfaces: The Free Area Theory Biophys. J., December 1, 2005; 89(6): 3997 - 4005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhou and R. M. Raphael Effect of Salicylate on the Elasticity, Bending Stiffness, and Strength of SOPC Membranes Biophys. J., September 1, 2005; 89(3): 1789 - 1801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Vlahakis and R. D. Hubmayr Cellular Stress Failure in Ventilator-injured Lungs Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1328 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Ayton, J. L. McWhirter, P. McMurtry, and G. A. Voth Coupling Field Theory with Continuum Mechanics: A Simulation of Domain Formation in Giant Unilamellar Vesicles Biophys. J., June 1, 2005; 88(6): 3855 - 3869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tarek Membrane Electroporation: A Molecular Dynamics Simulation Biophys. J., June 1, 2005; 88(6): 4045 - 4053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Wiggins and R. Phillips Membrane-Protein Interactions in Mechanosensitive Channels Biophys. J., February 1, 2005; 88(2): 880 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Leduc, O. Campas, K. B. Zeldovich, A. Roux, P. Jolimaitre, L. Bourel-Bonnet, B. Goud, J.-F. Joanny, P. Bassereau, and J. Prost From the Cover: Cooperative extraction of membrane nanotubes by molecular motors PNAS, December 7, 2004; 101(49): 17096 - 17101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Liu, T. Yang, M. J. Bruno, O. S. Andersen, and S. A. Simon Voltage-Gated Ion Channels in Nociceptors: Modulation by cGMP J Neurophysiol, October 1, 2004; 92(4): 2323 - 2332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Chen, R. W. Roberts, and J. W. Szostak The Emergence of Competition Between Model Protocells Science, September 3, 2004; 305(5689): 1474 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. V. Ly and M. L. Longo The Influence of Short-Chain Alcohols on Interfacial Tension, Mechanical Properties, Area/Molecule, and Permeability of Fluid Lipid Bilayers Biophys. J., August 1, 2004; 87(2): 1013 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Moshelion, N. Moran, and F. Chaumont Dynamic Changes in the Osmotic Water Permeability of Protoplast Plasma Membrane Plant Physiology, August 1, 2004; 135(4): 2301 - 2317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Girard, J. Pecreaux, G. Lenoir, P. Falson, J.-L. Rigaud, and P. Bassereau A New Method for the Reconstitution of Membrane Proteins into Giant Unilamellar Vesicles Biophys. J., July 1, 2004; 87(1): 419 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Cowan, E. M. Olivastro, D. E. Koppel, C. A. Loshon, B. Setlow, and P. Setlow Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile PNAS, May 18, 2004; 101(20): 7733 - 7738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. L. Lau, D. S. Ege, J. D. Lear, D. A. Hammer, and W. F. DeGrado Oligomerization of Fusogenic Peptides Promotes Membrane Fusion by Enhancing Membrane Destabilization Biophys. J., January 1, 2004; 86(1): 272 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Evans, V. Heinrich, F. Ludwig, and W. Rawicz Dynamic Tension Spectroscopy and Strength of Biomembranes Biophys. J., October 1, 2003; 85(4): 2342 - 2350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Patty and B. J. Frisken The Pressure-Dependence of the Size of Extruded Vesicles Biophys. J., August 1, 2003; 85(2): 996 - 1004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Shoemaker and T. K. Vanderlick Material Studies of Lipid Vesicles in the L{alpha} and L{alpha}-Gel Coexistence Regimes Biophys. J., February 1, 2003; 84(2): 998 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tristram-Nagle, Y. Liu, J. Legleiter, and J. F. Nagle Structure of Gel Phase DMPC Determined by X-Ray Diffraction Biophys. J., December 1, 2002; 83(6): 3324 - 3335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mally, J. Majhenc, S. Svetina, and B. Zeks Mechanisms of Equinatoxin II-Induced Transport through the Membrane of a Giant Phospholipid Vesicle Biophys. J., August 1, 2002; 83(2): 944 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shmeeda, P. Kaspler, J. Shleyer, R. Honen, M. Horowitz, and Y. Barenholz Heat acclimation in rats: modulation via lipid polyunsaturation Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2002; 283(2): R389 - R399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ayton, A. M. Smondyrev, S. G. Bardenhagen, P. McMurtry, and G. A. Voth Interfacing Molecular Dynamics and Macro-Scale Simulations for Lipid Bilayer Vesicles Biophys. J., August 1, 2002; 83(2): 1026 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Marsh Polarity and permeation profiles in lipid membranes PNAS, July 3, 2001; 98(14): 7777 - 7782. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
