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Biophys J, October 2000, p. 1761-1770, Vol. 79, No. 4
and
*Center for Studies in Physics and Biology, The Rockefeller
University, 1230 York Avenue, New York, NY 10021-6399; and
Cell Biology and Metabolism Branch, National Institute of
Child Health and Human Development, Bethesda, MD 20892 USA
A continuum description for diffusion in a simple model
for an inhomogeneous but isotropic media is derived and implemented numerically. The locally averaged density of diffusible marker is input
from experiment to define the sample. Then a single additional parameter, the effective diffusion constant, permits the quantitative simulation of diffusive relaxation from any initial condition. Using
this simulation, it is possible to model the recovery of a
fluorescently tagged protein in the endoplasmic reticulum (ER) after
photobleaching a substantial region of a live cell, and fit an
effective diffusion constant which is a property both of the geometry
of the ER and the marker. Such quantitative measurements permit
inferences about the topology and internal organization of this organelle.
Biophys J, October 2000, p. 1761-1770, Vol. 79, No. 4
© 2000 by the Biophysical Society 0006-3495/00/10/1761/10 $2.00
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