help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, L.
Right arrow Articles by Huang, H. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, L.
Right arrow Articles by Huang, H. W.

Biophys J, October 2000, p. 2002-2009, Vol. 79, No. 4

Crystallization of Antimicrobial Pores in Membranes: Magainin and Protegrin

Lin Yang,* Thomas M. Weiss,* Robert I. Lehrer,dagger and Huey W. Huang*

 *Physics Department, Rice University, Houston, Texas 77251-1892; and  dagger Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095 USA

Membrane pores spontaneously formed by antimicrobial peptides in membranes were crystallized for the first time by manipulating the sample hydration and temperature. Neutron diffraction shows that magainins and protegrins form stable pores in fully hydrated fluid membranes. At lower hydration levels or low temperature, the membrane multilayers crystallize. In one crystalline phase, the pores in each bilayer arrange in a regular hexagonal array and the bilayers are stacked into a hexagonal ABC lattice, corresponding to the cubic close-packed structure of spheres. In another crystalline phase, the bilayers are modulated into the rippled multilamellae, corresponding to a 2D monoclinic lattice. The phase diagrams are described. Crystallization of the membrane pores provides possibilities for diffraction studies that might provide useful information on the pore structures.

Biophys J, October 2000, p. 2002-2009, Vol. 79, No. 4
© 2000 by the Biophysical Society   0006-3495/00/10/2002/08  $2.00


This article has been cited by other articles:


Home page
 Biophys. JHome page
S. Qian, W. Wang, L. Yang, and H. W. Huang
Structure of the Alamethicin Pore Reconstructed by X-Ray Diffraction Analysis
Biophys. J., May 1, 2008; 94(9): 3512 - 3522.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
D. Tran, P. Tran, K. Roberts, G. Osapay, J. Schaal, A. Ouellette, and M. E. Selsted
Microbicidal Properties and Cytocidal Selectivity of Rhesus Macaque Theta Defensins
Antimicrob. Agents Chemother., March 1, 2008; 52(3): 944 - 953.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
H. K. Fung, C. A. Floudas, M. S. Taylor, L. Zhang, and D. Morikis
Toward Full-Sequence De Novo Protein Design with Flexible Templates for Human Beta-Defensin-2
Biophys. J., January 15, 2008; 94(2): 584 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. Shental-Bechor, T. Haliloglu, and N. Ben-Tal
Interactions of Cationic-Hydrophobic Peptides with Lipid Bilayers: A Monte Carlo Simulation Method
Biophys. J., September 15, 2007; 93(6): 1858 - 1871.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. Constantin, G. Brotons, A. Jarre, C. Li, and T. Salditt
Interaction of Alamethicin Pores in DMPC Bilayers
Biophys. J., June 1, 2007; 92(11): 3978 - 3987.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y.-L. Pan, J. T.-J. Cheng, J. Hale, J. Pan, R. E. W. Hancock, and S. K. Straus
Characterization of the Structure and Membrane Interaction of the Antimicrobial Peptides Aurein 2.2 and 2.3 from Australian Southern Bell Frogs
Biophys. J., April 15, 2007; 92(8): 2854 - 2864.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
W. Wang, L. Yang, and H. W. Huang
Evidence of Cholesterol Accumulated in High Curvature Regions: Implication to the Curvature Elastic Energy for Lipid Mixtures
Biophys. J., April 15, 2007; 92(8): 2819 - 2830.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Bakas, A. Chanturiya, V. Herlax, and J. Zimmerberg
Paradoxical Lipid Dependence of Pores Formed by the Escherichia coli {alpha}-Hemolysin in Planar Phospholipid Bilayer Membranes
Biophys. J., November 15, 2006; 91(10): 3748 - 3755.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Mani, S. D. Cady, M. Tang, A. J. Waring, R. I. Lehrer, and M. Hong
Membrane-dependent oligomeric structure and pore formation of a beta-hairpin antimicrobial peptide in lipid bilayers from solid-state NMR
PNAS, October 31, 2006; 103(44): 16242 - 16247.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Tremouilhac, E. Strandberg, P. Wadhwani, and A. S. Ulrich
Synergistic Transmembrane Alignment of the Antimicrobial Heterodimer PGLa/Magainin
J. Biol. Chem., October 27, 2006; 281(43): 32089 - 32094.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
W. Wang, D. Pan, Y. Song, W. Liu, L. Yang, and H. W. Huang
Method of X-Ray Anomalous Diffraction for Lipid Structures
Biophys. J., July 15, 2006; 91(2): 736 - 743.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M.-T. Lee, W.-C. Hung, F.-Y. Chen, and H. W. Huang
Many-Body Effect of Antimicrobial Peptides: On the Correlation Between Lipid's Spontaneous Curvature and Pore Formation
Biophys. J., December 1, 2005; 89(6): 4006 - 4016.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. E. VanCompernolle, R. J. Taylor, K. Oswald-Richter, J. Jiang, B. E. Youree, J. H. Bowie, M. J. Tyler, J. M. Conlon, D. Wade, C. Aiken, et al.
Antimicrobial Peptides from Amphibian Skin Potently Inhibit Human Immunodeficiency Virus Infection and Transfer of Virus from Dendritic Cells to T Cells
J. Virol., September 15, 2005; 79(18): 11598 - 11606.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Pietiainen, M. Gardemeister, M. Mecklin, S. Leskela, M. Sarvas, and V. P. Kontinen
Cationic antimicrobial peptides elicit a complex stress response in Bacillus subtilis that involves ECF-type sigma factors and two-component signal transduction systems
Microbiology, May 1, 2005; 151(5): 1577 - 1592.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. F. Lopez, S. O. Nielsen, B. Ensing, P. B. Moore, and M. L. Klein
Structure and Dynamics of Model Pore Insertion into a Membrane
Biophys. J., May 1, 2005; 88(5): 3083 - 3094.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. Allende, S. A. Simon, and T. J. McIntosh
Melittin-Induced Bilayer Leakage Depends on Lipid Material Properties: Evidence for Toroidal Pores
Biophys. J., March 1, 2005; 88(3): 1828 - 1837.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Spaar, C. Munster, and T. Salditt
Conformation of Peptides in Lipid Membranes Studied by X-Ray Grazing Incidence Scattering
Biophys. J., July 1, 2004; 87(1): 396 - 407.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Bhargava and J. B. Feix
Membrane Binding, Structure, and Localization of Cecropin-Mellitin Hybrid Peptides: A Site-Directed Spin-Labeling Study
Biophys. J., January 1, 2004; 86(1): 329 - 336.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
B. B. Bonev, Y.-H. Lam, G. Anderluh, A. Watts, R. S. Norton, and F. Separovic
Effects of the Eukaryotic Pore-Forming Cytolysin Equinatoxin II on Lipid Membranes and the Role of Sphingomyelin
Biophys. J., April 1, 2003; 84(4): 2382 - 2392.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
M. R. Yeaman and N. Y. Yount
Mechanisms of Antimicrobial Peptide Action and Resistance
Pharmacol. Rev., March 1, 2003; 55(1): 27 - 55.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Yang and H. W. Huang
A Rhombohedral Phase of Lipid Containing a Membrane Fusion Intermediate Structure
Biophys. J., March 1, 2003; 84(3): 1808 - 1817.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
N. Wustneck, R. Wustneck, J. Perez-Gil, and U. Pison
Effects of Oligomerization and Secondary Structure on the Surface Behavior of Pulmonary Surfactant Proteins SP-B and SP-C
Biophys. J., March 1, 2003; 84(3): 1940 - 1949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Tam, Y.-A. Lu, and J.-L. Yang
Correlations of Cationic Charges with Salt Sensitivity and Microbial Specificity of Cystine-stabilized beta -Strand Antimicrobial Peptides
J. Biol. Chem., December 20, 2002; 277(52): 50450 - 50456.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Koprivnjak, A. Peschel, M. H. Gelb, N. S. Liang, and J. P. Weiss
Role of Charge Properties of Bacterial Envelope in Bactericidal Action of Human Group IIA Phospholipase A2 against Staphylococcus aureus
J. Biol. Chem., November 27, 2002; 277(49): 47636 - 47644.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
D. J. A. Netz, M. d. C. d. F. Bastos, and H.-G. Sahl
Mode of Action of the Antimicrobial Peptide Aureocin A53 from Staphylococcus aureus
Appl. Envir. Microbiol., November 1, 2002; 68(11): 5274 - 5280.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. M. Hoover, C. Boulegue, D. Yang, J. J. Oppenheim, K. Tucker, W. Lu, and J. Lubkowski
The Structure of Human Macrophage Inflammatory Protein-3alpha /CCL20. LINKING ANTIMICROBIAL AND CC CHEMOKINE RECEPTOR-6-BINDING ACTIVITIES WITH HUMAN beta -DEFENSINS
J. Biol. Chem., September 27, 2002; 277(40): 37647 - 37654.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. W. Tinoco, A. da Silva Jr., A. Leite, A. P. Valente, and F. C. L. Almeida
NMR Structure of PW2 Bound to SDS Micelles. A TRYPTOPHAN-RICH ANTICOCCIDIAL PEPTIDE SELECTED FROM PHAGE DISPLAY LIBRARIES
J. Biol. Chem., September 20, 2002; 277(39): 36351 - 36356.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
L. Yang and H. W. Huang
Observation of a Membrane Fusion Intermediate Structure
Science, September 13, 2002; 297(5588): 1877 - 1879.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Efron, A. Dagan, L. Gaidukov, H. Ginsburg, and A. Mor
Direct Interaction of Dermaseptin S4 Aminoheptanoyl Derivative with Intraerythrocytic Malaria Parasite Leading to Increased Specific Antiparasitic Activity in Culture
J. Biol. Chem., June 28, 2002; 277(27): 24067 - 24072.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Kustanovich, D. E. Shalev, M. Mikhlin, L. Gaidukov, and A. Mor
Structural Requirements for Potent Versus Selective Cytotoxicity for Antimicrobial Dermaseptin S4 Derivatives
J. Biol. Chem., May 3, 2002; 277(19): 16941 - 16951.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
B. Lopez-Garcia, E. Perez-Paya, and J. F. Marcos
Identification of Novel Hexapeptides Bioactive against Phytopathogenic Fungi through Screening of a Synthetic Peptide Combinatorial Library
Appl. Envir. Microbiol., May 1, 2002; 68(5): 2453 - 2460.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Dagan, L. Efron, L. Gaidukov, A. Mor, and H. Ginsburg
In Vitro Antiplasmodium Effects of Dermaseptin S4 Derivatives
Antimicrob. Agents Chemother., April 1, 2002; 46(4): 1059 - 1066.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
P. Lindholm, U. Goransson, S. Johansson, P. Claeson, J. Gullbo, R. Larsson, L. Bohlin, and A. Backlund
Cyclotides: A Novel Type of Cytotoxic Agents
Mol. Cancer Ther., April 1, 2002; 1(6): 365 - 369.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Basanez, J. Zhang, B. N. Chau, G. I. Maksaev, V. A. Frolov, T. A. Brandt, J. Burch, J. M. Hardwick, and J. Zimmerberg
Pro-apoptotic Cleavage Products of Bcl-xL Form Cytochrome c-conducting Pores in Pure Lipid Membranes
J. Biol. Chem., August 10, 2001; 276(33): 31083 - 31091.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Zhang, A. Rozek, and R. E. W. Hancock
Interaction of Cationic Antimicrobial Peptides with Model Membranes
J. Biol. Chem., September 14, 2001; 276(38): 35714 - 35722.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Biophysical Society.