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Biophys J, October 2000, p. 2162-2170, Vol. 79, No. 4
and
Departments of *Electrical Engineering;
Physiology and Dalton Cardiovascular Research Center,
University of Missouri-Columbia, Research Park, Columbia, Missouri
65211 USA
High-resolution measurement of membrane capacitance in
the whole-cell-recording configuration can be used to detect small changes in membrane surface area that accompany exocytosis and endocytosis. We have investigated the noise of membrane capacitance measurements to determine the fundamental limits of resolution in
actual cells in the whole-cell mode. Two previously overlooked sources
of noise are particularly evident at low frequencies. The first noise
source is accompanied by a correlation between capacitance estimates,
whereas the second noise source is due to "1/f-like"
current noise. An analytic expression that summarizes the noise from
thermal and 1/f sources is derived, which agrees with
experimental measurements from actual cells over a large frequency
range. Our results demonstrate that the optimal frequencies for
capacitance measurements are higher than previously believed. Finally,
we demonstrate that the capacitance noise at high frequencies can be
reduced by compensating for the voltage drop of the sine wave across
the series resistance.
Biophys J, October 2000, p. 2162-2170, Vol. 79, No. 4
© 2000 by the Biophysical Society 0006-3495/00/10/2162/09 $2.00
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