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Biophys J, October 2000, p. 2199-2208, Vol. 79, No. 4
and
*Department of Biochemistry, The Weill Medical College of Cornell
University and Sloan-Kettering Institute, Memorial
Sloan-Kettering Cancer Center, New York, New York 10021 USA
Genetically encoded reporters for optical measurements of
presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We
have characterized pH-sensitive green fluorescent protein-based sensors
(pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins
have a pK ~ 7.1, which make them ideal for tracking synaptic
vesicle lumen pH upon cycling through the plasma membrane during action
potentials. A theoretical analysis of the expected signals using this
approach and guidelines for future reporter development are provided.
Biophys J, October 2000, p. 2199-2208, Vol. 79, No. 4
© 2000 by the Biophysical Society 0006-3495/00/10/2199/10 $2.00
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