| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophys J, December 2000, p. 3164-3171, Vol. 79, No. 6
*Department of Biochemistry and Cell Biology, and Institute of Biomembranes, Utrecht University, 3508 TD Utrecht; and §Department of Science of Food of Animal Origin, Utrecht University, 3508 TD, Utrecht, The Netherlands
A pressure-driven captive bubble surfactometer was used
to determine the role of surfactant proteins in refinement of the surface film. The advantage of this apparatus is that surface films can
be spread at the interface of an air bubble with a different lipid/protein composition than the subphase vesicles. Using different combinations of subphase vesicles and spread surface films a clear correlation between dipalmitoylphosphatidylcholine (DPPC) content and
minimum surface tension was observed. Spread phospholipid films
containing 50% DPPC over a subphase containing 50% DPPC vesicles did
not form stable surface films with a low minimum surface tension.
Addition of surfactant protein B (SP-B) to the surface film led to a
progressive decrease in minimum surface tension toward 1 mN/m upon
cycling, indicating an enrichment in DPPC. Surfactant protein C (SP-C)
had no such detectable refining effect on the film. Surfactant protein
A (SP-A) had a positive effect on refinement when it was present in the
subphase. However, this effect was only observed when SP-A was combined
with SP-B and incubated with subphase vesicles before addition to the
air bubble containing sample chamber. Comparison of spread films with adsorbed films indicated that refinement induced by SP-B occurs by
selective removal of non-DPPC lipids upon cycling. SP-A, combined with
SP-B, induces a selective adsorption of DPPC from subphase vesicles
into the surface film. This is achieved by formation of large lipid
structures which might resemble tubular myelin.
Biophys J, December 2000, p. 3164-3171, Vol. 79, No. 6
© 2000 by the Biophysical Society 0006-3495/00/12/3164/08 $2.00
This article has been cited by other articles:
 |
|
 |
|
  M. Saleem, M. C. Meyer, D. Breitenstein, and H.-J. Galla The Surfactant Peptide KL4 in Lipid Monolayers: PHASE BEHAVIOR, TOPOGRAPHY, AND CHEMICAL DISTRIBUTION J. Biol. Chem., February 22, 2008; 283(8): 5195 - 5207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Morrow, S. Temple, J. Stewart, and K. M. W. Keough Comparison of DPPC and DPPG Environments in Pulmonary Surfactant Models Biophys. J., July 1, 2007; 93(1): 164 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Saenz, O. Canadas, L. A. Bagatolli, F. Sanchez-Barbero, M. E. Johnson, and C. Casals Effect of Surfactant Protein A on the Physical Properties and Surface Activity of KL4-Surfactant Biophys. J., January 15, 2007; 92(2): 482 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Seurynck, N. J. Brown, C. W. Wu, K. W. Germino, E. K. Kohlmeir, E. P. Ingenito, M. R. Glucksberg, A. E. Barron, and M. Johnson Optical monitoring of bubble size and shape in a pulsating bubble surfactometer J Appl Physiol, August 1, 2005; 99(2): 624 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Rau, G. Vieten, J. J. Haitsma, J. Freihorst, C. Poets, B. M. Ure, and W. Bernhard Surfactant in Newborn Compared with Adolescent Pigs: Adaptation to Neonatal Respiration Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 694 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cruz, L. Vazquez, M. Velez, and J. Perez-Gil Effect of Pulmonary Surfactant Protein SP-B on the Micro- and Nanostructure of Phospholipid Films Biophys. J., January 1, 2004; 86(1): 308 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Morrow, N. Abu-Libdeh, J. Stewart, and K. M. W. Keough Interaction of Pulmonary Surfactant Protein SP-A with DPPC/Egg-PG Bilayers Biophys. J., October 1, 2003; 85(4): 2397 - 2405. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
