help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 8: 65-81 (1968)
© 1968 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dawid, I. B.
Right arrow Articles by Wolstenholme, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dawid, I. B.
Right arrow Articles by Wolstenholme, D. R.

Renaturation and Hybridization Studies of Mitochondrial DNA

Igor B. Dawid and David R. Wolstenholme

ABSTRACT

The products of the renaturation reaction of mitochondrial DNA from oocytes of Xenopus laevis have been studied by electron microscopy and CsCl equilibrium density gradient centrifugation. The reaction leads to the formation of intermediates containing single-stranded and double-stranded regions. Further reactions of these intermediates result in large complexes of interlinking double-stranded filaments. The formation of circular molecules of the same length as native circles of mitochondrial DNA was also observed. The formation of common high molecular weight complexes during joint reannealing of two DNA's with complementary sequences was used as a method to detect sequence homology in different DNA samples. Although this method does not produce quantitative data it offers several advantages in the present study. No homologies could be detected between the nuclear DNA and the mitochondrial DNA of X. laevis or of Rana pipiens. In interspecies comparisons homologies were found between the nuclear DNA's of X. laevis and the mouse and between the mitochondrial DNA's of X. laevis and the chick, but none between the mitochondrial DNA's of X. laevis and yeast. These results are interpreted as indicating the continuity of mitochondrial DNA during evolution.




This article has been cited by other articles:


Home page
 Cold Spring Harb Symp Quant BiolHome page
D. R. Wolstenholme, K. Koike, and P. Cochran-Fouts
Replication of Mitochondrial DNA: Replicative Forms of Molecules from Rat Tissues and Evidence for Discontinuous Replication
Cold Spring Harb Symp Quant Biol, January 1, 1974; 38(0): 267 - 280.
[Abstract] [PDF]

Home page
 ScienceHome page
M. M. K. Nass
Mitochondrial DNA: Advances, Problems, and Goals
Science, July 4, 1969; 165(3888): 25 - 35.
[PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1968 by the Biophysical Society.