Biophysical Journal 8: 1201-1210 (1968)
© 1968 the Biophysical Society

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The Complexing of Lysozyme with Poly C and Other homopolymers

ABSTRACT

Lysozyme forms very large complexes with poly C, in acetate buffer solutions (pH 5.4), when the ratio of lysozyme to poly C concentration is 3/2. When this is less than 3/8 there is virtually no complexing, as evidenced by the low light-scattering power of such mixtures. At such relatively high poly C concentrations, the addition of pancreatic ribonuclease causes both the intensity and dissymmetry of scattering to rise to very high values after which time the intensity falls exponentially with time and with very little change in dissymmetry. Other homopolymers also form largest complexes with lysozyme at characteristic concentration ratios.