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Biophysical Journal 8: 153-163 (1968)
© 1968 the Biophysical Society

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The Wavelength Dependence of Some Effects of Ultraviolet Radiation on In Vitro DNA of Phage {alpha}

M. Cremonese

ABSTRACT

The wavelength dependence of some of the effects of ultraviolet radiation on the physicochemical properties of DNA of phage {alpha} irradiated in vitro are discussed. An analytical ultracentrifuge and a spectrophotometer were used to study (a) the breaking of individual polynucleotide strands; (b) the local denaturation; (c) the presence of a fraction of molecules resistant to denaturation; and (d) the increase in the buoyant density of irradiated DNA. All the curves show a slight variation of the radiation efficiency in the range 2600-2800 A, and a well defined peak at {lambda} = 2880 A.






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Copyright © 1968 by the Biophysical Society.