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Biophys J, January 2001, p. 241-253, Vol. 80, No. 1

KCNKØ: Single, Cloned Potassium Leak Channels Are Multi-Ion Pores

Nitza Ilan and Steve A. N. Goldstein

Departments of Pediatrics and Cellular and Molecular Physiology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 USA

KCNKØ was the first clone to show attributes of a leak conductance: voltage-independent potassium currents that develop without delay. Its novel product is predicted to have two nonidentical P domains and four transmembrane segments and to assemble in pairs. Here, the mechanistic basis for leak is examined at the single-channel level. KCNKØ channels open at all voltages in bursts that last for minutes with open probability close to 1. The channels also enter a minutes-long closed state in a tightly regulated fashion. KCNKØ has a common relative permeability series (Eisenman type IV) but conducts only thallium and potassium readily. KCNKØ exhibits concentration-dependent unitary conductance, anomalous mole fraction behavior, and pore occlusion by barium. These observations argue for ion-channel and ion-ion interactions in a multi-ion pore and deny the operation of independence or constant-field current formulations. Despite their unique function and structure, leakage channels are observed to operate like classical potassium channels formed with one-P-domain subunits.

Biophys J, January 2001, p. 241-253, Vol. 80, No. 1
© 2001 by the Biophysical Society   0006-3495/01/01/241/13  $2.00


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