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Biophys J, February 2001, p. 626-634, Vol. 80, No. 2

Control of Glycolytic Dynamics by Hexose Transport in Saccharomyces cerevisiae

Karin A. Reijenga,* Jacky L. Snoep,*dagger Jasper A. Diderich,Dagger Henk W. van Verseveld,* Hans V. Westerhoff,*Dagger and Bas TeusinkDagger

 *Department of Molecular Cell Physiology, BioCentrum Amsterdam, Faculty of Biology, Vrije Universiteit, NL-1081 HV Amsterdam, The Netherlands;  dagger Department of Biochemistry, University of Stellenbosch, Matieland 7602, Stellenbosch, South Africa; and  Dagger Swammerdam Institute for Life Science, BioCentrum Amsterdam, University of Amsterdam, NL-1018 TV Amsterdam, The Netherlands

It is becoming accepted that steady-state fluxes are not necessarily controlled by single rate-limiting steps. This leaves open the issue whether cellular dynamics are controlled by single pacemaker enzymes, as has often been proposed. This paper shows that yeast sugar transport has substantial but not complete control of the frequency of glycolytic oscillations. Addition of maltose, a competitive inhibitor of glucose transport, reduced both average glucose consumption flux and frequency of glycolytic oscillations. Assuming a single kinetic component and a symmetrical carrier, a frequency control coefficient of between 0.4 and 0.6 and an average-flux control coefficient of between 0.6 and 0.9 were calculated for hexose transport activity. In a second approach, mannose was used as the carbon and free-energy source, and the dependencies on the extracellular mannose concentration of the transport activity, of the frequency of oscillations, and of the average flux were compared. In this case the frequency control coefficient and the average-flux control coefficient of hexose transport activity amounted to 0.7 and 0.9, respectively. From these results, we conclude that 1) transport is highly important for the dynamics of glycolysis, 2) most but not all control resides in glucose transport, and 3) there should at least be one step other than transport with substantial control.

Biophys J, February 2001, p. 626-634, Vol. 80, No. 2
© 2001 by the Biophysical Society   0006-3495/01/02/626/09  $2.00


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