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Biophys J, February 2001, p. 874-881, Vol. 80, No. 2
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, Minnesota 55108 USA
When a single molecule of double-stranded DNA is
stretched beyond its B-form contour length, the measured force shows a
highly cooperative overstretching transition. We have investigated the source of this transition by altering helix stability with solution pH.
As solution pH was increased from pH 6.0 to pH 10.6 in 250 mM NaCl, the
overstretching transition force decreased from 67.0 ± 0.8 pN to
56.2 ± 0.8 pN, whereas the transition width remained nearly
constant. As the pH was lowered from pH 6.0 to pH 3.1, the
overstretching force decreased from 67.0 ± 0.8 pN to 47.0 ± 1.0 pN, but the transition width increased from 3.0 ± 0.6 pN to
16.0 ± 3 pN. These results quantitatively agree with a model that
asserts that DNA strand dissociation, or melting, occurs during the
overstretching transition.
Biophys J, February 2001, p. 874-881, Vol. 80, No. 2
© 2001 by the Biophysical Society 0006-3495/01/02/874/08 $2.00
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