Biophys J, March 2001, p. 1088-1103, Vol. 80, No. 3

Bayesian Restoration of Ion Channel Records using Hidden Markov Models

Rafael Rosales,^* J. Alex Stark, William J. Fitzgerald, and Stephen B. Hladky^*

 ^*Pharmacology and  Engineering, University of Cambridge, Cambridge CB2 1QJ, United Kingdom

Hidden Markov models have been used to restore recorded signals of single ion channels buried in background noise. Parameter estimation and signal restoration are usually carried out through likelihood maximization by using variants of the Baum-Welch forward-backward procedures. This paper presents an alternative approach for dealing with this inferential task. The inferences are made by using a combination of the framework provided by Bayesian statistics and numerical methods based on Markov chain Monte Carlo stochastic simulation. The reliability of this approach is tested by using synthetic signals of known characteristics. The expectations of the model parameters estimated here are close to those calculated using the Baum-Welch algorithm, but the present methods also yield estimates of their errors. Comparisons of the results of the Bayesian Markov Chain Monte Carlo approach with those obtained by filtering and thresholding demonstrate clearly the superiority of the new methods.

Biophys J, March 2001, p. 1088-1103, Vol. 80, No. 3
© 2001 by the Biophysical Society   0006-3495/01/03/1088/16  $2.00

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