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Biophys J, April 2001, p. 1733-1743, Vol. 80, No. 4
and
*The Department of Chemical Engineering, Ohio University, Athens,
Ohio 45701 and The Department of Biomedical Engineering,
The University of Memphis, Memphis, Tennessee 38152, USA
The diameter of circulating cells that may adhere to the
vascular endothelium spans an order of magnitude from ~2 µm (e.g., platelets) to ~20 µm (e.g., a metastatic cell). Although
mathematical models indicate that the adhesion exhibited by a cell will
be a function of cell diameter, there have been few experimental investigations into the role of cell diameter in adhesion. Thus, in
this study, we coated 5-, 10-, 15-, and 20-µm-diameter microspheres with the recombinant P-selectin glycoprotein ligand
1 construct 19.ek.Fc. We compared the adhesion of the 19.ek.Fc microspheres to
P-selectin under in vitro flow conditions. We found that 1) at
relatively high shear, the rate of attachment of the 19.ek.Fc microspheres decreased with increasing microsphere diameter whereas, at
a lower shear, the rate of attachment was not affected by the microsphere diameter; 2) the shear stress required to set in motion a
firmly adherent 19.ek.Fc microsphere decreased with increasing microsphere diameter; and 3) the rolling velocity of the 19.ek.Fc microspheres increased with increasing microsphere diameter. These results suggest that attachment, rolling, and firm adhesion are functions of particle diameter and provide experimental proof for
theoretical models that indicate a role for cell diameter in adhesion.
Biophys J, April 2001, p. 1733-1743, Vol. 80, No. 4
© 2001 by the Biophysical Society 0006-3495/01/04/1733/11 $2.00
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