| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophys J, April 2001, p. 1819-1828, Vol. 80, No. 4
and
*Biophysics and Structural Biology Graduate Group, Section of
Molecular and Cellular Biology, University of California, Davis, Davis,
California 95616 USA; Department of Pharmaceutics, The
Royal Danish School of Pharmacy, DK-2100, Copenhagen, Denmark; and
Department of Chemistry, Technical University of
Denmark, DK-2800, Lyngby, Denmark
The thermodynamic phase behavior and lateral lipid
membrane organization of unilamellar vesicles made from mixtures of
1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and
1,2 distearoyl-sn-glycero-3-phosphocholine (DSPC) were investigated by fluorescence resonance energy transfer (FRET) as a
function of temperature and composition. This was done by incorporating
a headgroup-labeled lipid donor (NBD-DPPE) and acceptor (N-Rh-DPPE) in
low concentrations into the binary mixtures. Two instances of increased
energy transfer efficiency were observed close to the phase lines in
the DMPC/DSPC phase diagram. The increase in energy transfer efficiency
was attributed to a differential preference of the probes for dynamic
and fluctuating gel/fluid coexisting phases. This differential
preference causes the probes to segregate (S. Pedersen, K. Jørgensen,
T. R. Baekmark, and O. G. Mouritsen, 1996, Biophys.
J. 71:554-560). The observed increases in energy transfer
match with the boundaries of the DMPC/DSPC phase diagram, as measured
by Fourier transform infrared spectroscopy (FTIR) and differential
scanning calorimetry (DSC). We propose that the two instances of probe
segregation are due to the presence of DMPC-rich and DSPC-rich domains,
which form a dynamic structure of gel/fluid coexisting phases at two
different temperatures. Monitoring the melting profile of each lipid
component independently by FTIR shows that the domain structure is
formed by DMPC-rich and DSPC-rich domains rather than by pure DMPC and
DSPC domains.
Biophys J, April 2001, p. 1819-1828, Vol. 80, No. 4
© 2001 by the Biophysical Society 0006-3495/01/04/1819/10 $2.00
This article has been cited by other articles:
 |
|
 |
|
  M. Fidorra, L. Duelund, C. Leidy, A. C. Simonsen, and L.A. Bagatolli Absence of Fluid-Ordered/Fluid-Disordered Phase Coexistence in Ceramide/POPC Mixtures Containing Cholesterol Biophys. J., June 15, 2006; 90(12): 4437 - 4451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Leidy, L. Linderoth, T. L. Andresen, O. G. Mouritsen, K. Jorgensen, and G. H. Peters Domain-Induced Activation of Human Phospholipase A2 Type IIA: Local versus Global Lipid Composition Biophys. J., May 1, 2006; 90(9): 3165 - 3175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Tricerri, J. D. Toledo, S. A. Sanchez, T. L. Hazlett, E. Gratton, A. Jonas, and H. A. Garda Visualization and analysis of apolipoprotein A-I interaction with binary phospholipid bilayers J. Lipid Res., April 1, 2005; 46(4): 669 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Redfern and A. Gericke pH-dependent domain formation in phosphatidylinositol polyphosphate/phosphatidylcholine mixed vesicles J. Lipid Res., March 1, 2005; 46(3): 504 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Redfern and A. Gericke Domain Formation in Phosphatidylinositol Monophosphate/Phosphatidylcholine Mixed Vesicles Biophys. J., May 1, 2004; 86(5): 2980 - 2992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Giocondi and C. Le Grimellec Temperature Dependence of the Surface Topography in Dimyristoylphosphatidylcholine/Distearoylphosphatidylcholine Multibilayers Biophys. J., April 1, 2004; 86(4): 2218 - 2230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Silvius Fluorescence Energy Transfer Reveals Microdomain Formation at Physiological Temperatures in Lipid Mixtures Modeling the Outer Leaflet of the Plasma Membrane Biophys. J., August 1, 2003; 85(2): 1034 - 1045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Cannon, G. Heath, J. Huang, P. Somerharju, J. A. Virtanen, and K. H. Cheng Time-Resolved Fluorescence and Fourier Transform Infrared Spectroscopic Investigations of Lateral Packing Defects and Superlattice Domains in Compositionally Uniform Cholesterol/Phosphatidylcholine Bilayers Biophys. J., June 1, 2003; 84(6): 3777 - 3791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Ricker, N. M. Tsvetkova, W. F. Wolkers, C. Leidy, F. Tablin, M. Longo, and J. H. Crowe Trehalose Maintains Phase Separation in an Air-Dried Binary Lipid Mixture Biophys. J., May 1, 2003; 84(5): 3045 - 3051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Leidy, T. Kaasgaard, J. H. Crowe, O. G. Mouritsen, and K. Jorgensen Ripples and the Formation of Anisotropic Lipid Domains: Imaging Two-Component Supported Double Bilayers by Atomic Force Microscopy Biophys. J., November 1, 2002; 83(5): 2625 - 2633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. I. Michonova-Alexova and I. P. Sugar Component and State Separation in DMPC/DSPC Lipid Bilayers: A Monte Carlo Simulation Study Biophys. J., October 1, 2002; 83(4): 1820 - 1833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fahsel, E.-M. Pospiech, M. Zein, T. L. Hazlet, E. Gratton, and R. Winter Modulation of Concentration Fluctuations in Phase-Separated Lipid Membranes by Polypeptide Insertion Biophys. J., July 1, 2002; 83(1): 334 - 344. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
