help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Condorelli, P.
Right arrow Articles by George, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Condorelli, P.
Right arrow Articles by George, S. C.

Biophys J, May 2001, p. 2110-2119, Vol. 80, No. 5

In Vivo Control of Soluble Guanylate Cyclase Activation by Nitric Oxide: A Kinetic Analysis

Peter Condorelli* and Steven C. George*dagger

 *Department of Chemical and Biochemical Engineering and Materials Science,  dagger The Center for Biomedical Engineering, University of California, Irvine, Irvine, California 92697-2575 USA

Free nitric oxide (NO) activates soluble guanylate cyclase (sGC), an enzyme, within both pulmonary and vascular smooth muscle. sGC catalyzes the cyclization of guanosine 5'-triphosphate to guanosine 3',5'-cyclic monophosphate (cGMP). Binding rates of NO to the ferrous heme(s) of sGC have been measured in vitro. However, a missing link in our understanding of the control mechanism of sGC by NO is a comprehensive in vivo kinetic analysis. Available literature data suggests that NO dissociation from the heme center of sGC is accelerated by its interaction with one or more cofactors in vivo. We present a working model for sGC activation and NO consumption in vivo. Our model predicts that NO influences the cGMP formation rate over a concentration range of approx 5-100 nM (apparent Michaelis constant approx  23 nM), with Hill coefficients between 1.1 and 1.5. The apparent reaction order for NO consumption by sGC is dependent on NO concentration, and varies between 0 and 1.5. Finally, the activation of sGC (half-life approx  1-2 s) is much more rapid than deactivation (approx 50 s). We conclude that control of sGC in vivo is most likely ultra-sensitive, and that activation in vivo occurs at lower NO concentrations than previously reported.

Biophys J, May 2001, p. 2110-2119, Vol. 80, No. 5
© 2001 by the Biophysical Society   0006-3495/01/05/2110/10  $2.00


This article has been cited by other articles:


Home page
 HypertensionHome page
J. D. Humphrey
Mechanisms of Arterial Remodeling in Hypertension: Coupled Roles of Wall Shear and Intramural Stress
Hypertension, August 1, 2008; 52(2): 195 - 200.
[Full Text] [PDF]

Home page
 Biophys. JHome page
X. Liu, P. Srinivasan, E. Collard, P. Grajdeanu, J. L. Zweier, and A. Friedman
Nitric Oxide Diffusion Rate is Reduced in the Aortic Wall
Biophys. J., March 1, 2008; 94(5): 1880 - 1889.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Martin, V. Berka, E. Bogatenkova, F. Murad, and A.-L. Tsai
Ligand Selectivity of Soluble Guanylyl Cyclase: EFFECT OF THE HYDROGEN-BONDING TYROSINE IN THE DISTAL HEME POCKET ON BINDING OF OXYGEN, NITRIC OXIDE, AND CARBON MONOXIDE
J. Biol. Chem., September 22, 2006; 281(38): 27836 - 27845.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H.-W. Shin, P. Condorelli, and S. C. George
Examining axial diffusion of nitric oxide in the lungs using heliox and breath hold
J Appl Physiol, February 1, 2006; 100(2): 623 - 630.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H.-W. Shin, P. Condorelli, and S. C. George
A new and more accurate technique to characterize airway nitric oxide using different breath-hold times
J Appl Physiol, May 1, 2005; 98(5): 1869 - 1877.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. R. Hyduke and J. C. Liao
Analysis of nitric oxide donor effectiveness in resistance vessels
Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2390 - H2399.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Balashova, F.-J. Chang, M. Lamothe, Q. Sun, and A. Beuve
Characterization of a Novel Type of Endogenous Activator of Soluble Guanylyl Cyclase
J. Biol. Chem., January 21, 2005; 280(3): 2186 - 2196.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
N. M. Tsoukias, M. Kavdia, and A. S. Popel
A theoretical model of nitric oxide transport in arterioles: frequency- vs. amplitude-dependent control of cGMP formation
Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1043 - H1056.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. Condorelli and S. C. George
Free nitric oxide diffusion in the bronchial microcirculation
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2660 - H2670.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. C. Bellamy, J. Wood, and J. Garthwaite
On the activation of soluble guanylyl cyclase by nitric oxide
PNAS, January 8, 2002; 99(1): 507 - 510.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Kavdia, N. M. Tsoukias, and A. S. Popel
Model of nitric oxide diffusion in an arteriole: impact of hemoglobin-based blood substitutes
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2245 - H2253.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Biophysical Society.