help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fiorini, M.
Right arrow Articles by Abell, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fiorini, M.
Right arrow Articles by Abell, C.

Biophys J, May 2001, p. 2471-2476, Vol. 80, No. 5

Chemical Force Microscopy with Active Enzymes

Maria Fiorini, Rachel McKendry, Matthew A. Cooper, Trevor Rayment, and Christopher Abell

Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom

The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.

Biophys J, May 2001, p. 2471-2476, Vol. 80, No. 5
© 2001 by the Biophysical Society   0006-3495/01/05/2471/06  $2.00




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Biophysical Society.