Biophys J, June 2001, p. 2493-2504, Vol. 80, No. 6

Pore-to-Pore Hopping Model for the Interpretation of the Pulsed Gradient Spin Echo Attenuation of Water Diffusion in Cell Suspension Systems

Pang-Chih Jiang,^* Tsyr-Yan Yu,^* Wann-Cherng Perng, and Lian-Pin Hwang^*

 ^*Department of Chemistry, National Taiwan University and Institute of Atomic and Molecular Sciences, Academia Sinica, and  Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China

A simplified pore-to-pore hopping model for the two-phase diffusion problem is developed for the analysis of the pulsed gradient spin echo (PGSE) attenuation of water diffusion in the condensed cell suspension systems. In this model, the two phases inside and outside the cells are treated as two different kinds of pores, and the spin-bearing molecules perform hopping diffusion between them. The size and the orientations of those two respective pores are considered, and then the diffraction pattern of the PGSE attenuation may be well simulated. Nevertheless, the intensity of the characteristic peak decreases with increasing membrane permeability, from which the exchange time may be estimated. We then analyze the experimental ¹H PGSE results of the erythrocytes suspension system. The water-residence lifetime in the erythrocyte is obtained to be 10 ms, which is the same as that estimated from the two-region approximation. Furthermore, the PGSE attenuation curve of addition of p-Chloromercuribenzenesulfonate (p-CMBS) is also discussed. It predicts that the alignment of erythrocytes will become normal to the magnetic field direction after the addition of p-CMBS, and inspection using a light microscope confirms that result.

Biophys J, June 2001, p. 2493-2504, Vol. 80, No. 6
© 2001 by the Biophysical Society   0006-3495/01/06/2493/12  $2.00