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Biophys J, June 2001, p. 2775-2788, Vol. 80, No. 6

Ternary Phase Diagram of Dipalmitoyl-PC/Dilauroyl-PC/Cholesterol: Nanoscopic Domain Formation Driven by Cholesterol

Gerald W. Feigenson and Jeffrey T. Buboltz

Field of Biophysics, Biotechnology Building, Cornell University, Ithaca, New York 14853 USA

A ternary phase diagram is proposed for the hydrated lamellar lipid mixture dipalmitoylphosphatidylcholine/dilauroylphosphatidylcholine/cholesterol (DPPC/DLPC/cholesterol) at room temperature. The entire composition space has been thoroughly mapped by complementary experimental techniques, revealing interesting phase behavior that has not been previously described. Confocal fluorescence microscopy shows a regime of coexisting DPPC-rich ordered and DLPC-rich fluid lamellar phases, having an upper boundary at apparently constant cholesterol mole fraction chi chol ~ 0.16. Fluorescence resonance energy transfer experiments confirm the identification and extent of this two-phase regime and, furthermore, reveal a 1-phase regime between chi chol ~ 0.16 and 0.25, consisting of ordered and fluid nanoscopic domains. Dipyrene-PC excimer/monomer measurements confirm the new regime between chi chol ~ 0.16 and 0.25 and also show that rigidly ordered phases seem to disappear around chi chol ~ 0.25. This study should be considered as a step toward a more complete understanding of lateral heterogeneity within biomembranes. Cholesterol may play a role in domain separation on the nanometer scale.

Biophys J, June 2001, p. 2775-2788, Vol. 80, No. 6
© 2001 by the Biophysical Society   0006-3495/01/06/2775/14  $2.00


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