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Biophys J, June 2001, p. 2809-2822, Vol. 80, No. 6

Frequency-Dependent Distortion of Meridional Intensity Changes during Sinusoidal Length Oscillations of Activated Skeletal Muscle

Maria Angela Bagni,* Barbara Colombini,* Heinz Amenitsch,dagger Sigrid Bernstorff,dagger Christopher Charles Ashley,Dagger Gert Rapp,§ and Peter John GriffithsDagger

 *Dipartimento di Scienze Fisiologiche, Università degli Studi di Firenze, Florence I-50132, Italy;  dagger Sincrotrone Trieste S.C.p.A., I-34012 Basovizza TS, Italy;  Dagger University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, United Kingdom; and  §Hasylab at Deutsches Elekronen-Synekrotron, Gebäude 25f, D-22603 Hamburg, Germany

Bundles of intact, tetanized skeletal muscle fibers from Rana temporaria were subjected to sinusoidal length oscillations in the frequency domain 100 Hz to 3 kHz while measuring force and sarcomere length. Simultaneously, intensity of the third-order x-ray reflection of the axial myosin unit cell (IM3) was measured using synchrotron radiation. At oscillation frequencies <1 kHz, IM3 was distorted during the shortening phase of the sinusoid (i.e., where bundle length was less than rest length). Otherwise, during the stretch phase of oscillations at all frequencies, during the shortening phase of oscillations above 1 kHz, and for bundles in the rigor state, IM3 was approximately sinusoidal in form. Mean IM3 during oscillations was reduced by 20% compared to the isometric value, suggesting a possible change in S1 disposition during oscillations. However, the amplitude of length change required to produce distortion (estimated from the phase angle at which distortion was first evident) corresponded to that of a step release sufficient to reach the maximum IM3, indicating a mean S1 disposition during oscillations close to that during an isometric tetanus. The mechanical properties of the bundle during oscillations were also consistent with an unaltered S1 disposition during oscillations.

Biophys J, June 2001, p. 2809-2822, Vol. 80, No. 6
© 2001 by the Biophysical Society   0006-3495/01/06/2809/14  $2.00


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