help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krueger, B. P.
Right arrow Articles by van Grondelle, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krueger, B. P.
Right arrow Articles by van Grondelle, R.

Biophys J, June 2001, p. 2843-2855, Vol. 80, No. 6

Energy Transfer in the Peridinin Chlorophyll-a Protein of Amphidinium carterae Studied by Polarized Transient Absorption and Target Analysis

Brent P. Krueger,* Stefania S. Lampoura,* Ivo H. M. van Stokkum,* Emmanouil Papagiannakis,* Jante M. Salverda,* Claudiu C. Gradinaru,* Danielis Rutkauskas,* Roger G. Hiller,dagger and Rienk van Grondelle*

 *Department of Physics and Astronomy, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands; and  dagger School of Biological Sciences, Macquarie University, NSW 2109, Australia

The peridinin chlorophyll-a protein (PCP) of dinoflagellates differs from the well-studied light-harvesting complexes of purple bacteria and green plants in its large (4:1) carotenoid to chlorophyll ratio and the unusual properties of its primary pigment, the carotenoid peridinin. We utilized ultrafast polarized transient absorption spectroscopy to examine the flow of energy in PCP after initial excitation into the strongly allowed peridinin S2 state. Global and target analysis of the isotropic and anisotropic decays reveals that significant excitation (25-50%) is transferred to chlorophyll-a directly from the peridinin S2 state. Because of overlapping positive and negative features, this pathway was unseen in earlier single-wavelength experiments. In addition, the anisotropy remains constant and high in the peridinin population, indicating that energy transfer from peridinin to peridinin represents a minor or negligible pathway. The carotenoids are also coupled directly to chlorophyll-a via a low-lying singlet state S1 or the recently identified SCT. We model this energy transfer time scale as 2.3 ± 0.2 ps, driven by a coupling of ~47 cm-1. This coupling strength allows us to estimate that the peridinin S1/SCT donor state transition moment is ~3 D.

Biophys J, June 2001, p. 2843-2855, Vol. 80, No. 6
© 2001 by the Biophysical Society   0006-3495/01/06/2843/13  $2.00


This article has been cited by other articles:


Home page
 Biophys. JHome page
T. Polivka, T. Pascher, and R. G. Hiller
Energy Transfer in the Peridinin-Chlorophyll Protein Complex Reconstituted with Mixed Chlorophyll Sites
Biophys. J., April 15, 2008; 94(8): 3198 - 3207.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. T. A. Alexandre, D. C. Luhrs, I. H. M. van Stokkum, R. Hiller, M.-L. Groot, J. T. M. Kennis, and R. van Grondelle
Triplet State Dynamics in Peridinin-Chlorophyll-a-Protein: A New Pathway of Photoprotection in LHCs?
Biophys. J., September 15, 2007; 93(6): 2118 - 2128.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. Krikunova, H. Lokstein, D. Leupold, R. G. Hiller, and B. Voigt
Pigment-Pigment Interactions in PCP of Amphidinium carterae Investigated by Nonlinear Polarization Spectroscopy in the Frequency Domain
Biophys. J., January 1, 2006; 90(1): 261 - 271.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Gibasiewicz, R. Croce, T. Morosinotto, J. A. Ihalainen, I. H. M. van Stokkum, J. P. Dekker, R. Bassi, and R. van Grondelle
Excitation Energy Transfer Pathways in Lhca4
Biophys. J., March 1, 2005; 88(3): 1959 - 1969.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. S. Larsen, I. H. M. van Stokkum, M. Vengris, M. A. van der Horst, F. L. de Weerd, K. J. Hellingwerf, and R. van Grondelle
Incoherent Manipulation of the Photoactive Yellow Protein Photocycle with Dispersed Pump-Dump-Probe Spectroscopy
Biophys. J., September 1, 2004; 87(3): 1858 - 1872.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Zigmantas, R. G. Hiller, V. Sundstrom, and T. Polivka
Carotenoid to chlorophyll energy transfer in the peridinin-chlorophyll-a-protein complex involves an intramolecular charge transfer state
PNAS, December 24, 2002; 99(26): 16760 - 16765.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Biophysical Society.