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Biophys J, July 2001, p. 153-169, Vol. 81, No. 1

Interaction of Lung Surfactant Proteins with Anionic Phospholipids

D. Y. Takamoto,* M. M. Lipp,* A. von Nahmen,* Ka Yee C. Lee,dagger A. J. Waring,Dagger and J. A. Zasadzinski*

 *Department of Chemical Engineering, University of California, Santa Barbara, California 93106,  dagger Department of Chemistry, University of Chicago, Chicago, Illinois 60637, and  Dagger Department of Pediatrics, MLK/Drew University Medical Center and University of California, Los Angeles, California 90059 USA

Langmuir isotherms, fluorescence microscopy, and atomic force microscopy were used to study lung surfactant specific proteins SP-B and SP-C in monolayers of dipalmitoylphosphatidylglycerol (DPPG) and palmitoyloleoylphosphatidylglycerol (POPG), which are representative of the anionic lipids in native and replacement lung surfactants. Both SP-B and SP-C eliminate squeeze-out of POPG from mixed DPPG/POPG monolayers by inducing a two- to three-dimensional transformation of the fluid-phase fraction of the monolayer. SP-B induces a reversible folding transition at monolayer collapse, allowing all components of surfactant to remain at the interface during respreading. The folds remain attached to the monolayer, are identical in composition and morphology to the unfolded monolayer, and are reincorporated reversibly into the monolayer upon expansion. In the absence of SP-B or SP-C, the unsaturated lipids are irreversibly lost at high surface pressures. These morphological transitions are identical to those in other lipid mixtures and hence appear to be independent of the detailed lipid composition of the monolayer. Instead they depend on the more general phenomena of coexistence between a liquid-expanded and liquid-condensed phase. These three-dimensional monolayer transitions reconcile how lung surfactant can achieve both low surface tensions upon compression and rapid respreading upon expansion and may have important implications toward the optimal design of replacement surfactants. The overlap of function between SP-B and SP-C helps explain why replacement surfactants lacking in one or the other proteins often have beneficial effects.

Biophys J, July 2001, p. 153-169, Vol. 81, No. 1
© 2001 by the Biophysical Society   0006-3495/01/07/153/17  $2.00


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