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Biophys J, July 2001, p. 57-65, Vol. 81, No. 1

Membrane-Initiated Ca2+ Signals Are Reshaped during Propagation to Subcellular Regions

Werner J. H. Koopman,* Wim J. J. M. Scheenen,* Rachel J. Errington,dagger Dagger Peter H. G. M. Willems,dagger René J. M. Bindels,Dagger Eric W. Roubos,* and Bruce G. Jenks*

Departments of  *Cellular Animal Physiology,  dagger Biochemistry, and  Dagger Cell Physiology, University of Nijmegen, Nijmegen, The Netherlands

An important aspect of Ca2+ signaling is the ability of cells to generate intracellular Ca2+ waves. In this study we have analyzed the cellular and subcellular kinetics of Ca2+ waves in a neuroendocrine transducer cell, the melanotrope of Xenopus laevis, using the ratiometric Ca2+ probe indo-1 and video-rate UV confocal laser-scanning microscopy. The purpose of the present study was to investigate how local Ca2+ changes contribute to a global Ca2+ signal; subsequently we quantified how a Ca2+ wave is kinetically reshaped as it is propagated through the cell. The combined kinetics of all subcellular Ca2+ signals determined the shape of the total cellular Ca2+ signal, but each subcellular contribution to the cellular signal was not constant in time. Near the plasma membrane, [Ca2+]i increased and decreased rapidly, processes that can be described by a linear and exponential function, respectively. In more central parts of the cell slower kinetics were observed that were best described by a Hill equation. This reshaping of the Ca2+ wave was modeled with an equation derived from a low-pass RC filter. We propose that the differences in spatial kinetics of the Ca2+ signal serves as a mechanism by which the same cellular Ca2+ signal carries different regulatory information to different subcellular regions of the cell, thus evoking differential cellular responses.

Biophys J, July 2001, p. 57-65, Vol. 81, No. 1
© 2001 by the Biophysical Society   0006-3495/01/07/57/09  $2.00


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