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Biophys J, August 2001, p. 827-837, Vol. 81, No. 2

Voltage-Dependent Formation of Gramicidin Channels in Lipid Bilayers

John Sandblom, Juris Galvanovskis, and Barbro Jilderos

Department of Medical Biophysics, Göteborg University, 40530 Göteborg, Sweden

The formation kinetics of gramicidin A channels in lipid bilayer membranes has been characterized as a function of voltage for different solution conditions and membrane composition. The frequency of channel events was measured during the application of voltage ramps and counted in given intervals, a procedure that eliminated the effects of drift in gramicidin concentration. The formation rate was found to increase strongly with voltages up to ~50 mV and then to level off slightly. The shape of the voltage dependence was independent of lipid solvent and ramp speed but differed for different ions and different solution concentrations. This suggested an ion occupancy effect on the formation rate that was further supported by the fact that the minimum of the formation rate was shifted toward the equilibrium potential in asymmetric solution concentrations. The effects are explained in terms of a model that contains two contributions to the voltage dependence, a voltage-dependent ion binding to the monomers and a polarization of monomers by the applied electric field and by the occupied ions. The theory is found to give a good fit to experimental data.

Biophys J, August 2001, p. 827-837, Vol. 81, No. 2
© 2001 by the Biophysical Society   0006-3495/01/08/827/11  $2.00


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