help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, L.
Right arrow Articles by Huang, H. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, L.
Right arrow Articles by Huang, H. W.

Biophys J, September 2001, p. 1475-1485, Vol. 81, No. 3

Barrel-Stave Model or Toroidal Model? A Case Study on Melittin Pores

Lin Yang, Thad A. Harroun, Thomas M. Weiss, Lai Ding, and Huey W. Huang

Department of Physics and Astronomy, Rice University Houston, Texas 77251 USA

Transmembrane pores induced by amphiphilic peptides, including melittin, are often modeled with the barrel-stave model after the alamethicin pore. We examine this assumption on melittin by using two methods, oriented circular dichroism (OCD) for detecting the orientation of melittin helix and neutron scattering for detecting transmembrane pores. OCD spectra of melittin were systematically measured. Melittin can orient either perpendicularly or parallel to a lipid bilayer, depending on the physical condition and the composition of the bilayer. Transmembrane pores were detected when the helices oriented perpendicularly to the plane of the bilayers, not when the helices oriented parallel to the bilayers. The evidence that led to the barrel-stave model for alamethicin and that to the toroidal model for magainin were reviewed. The properties of melittin pores are closely similar to that of magainin but unlike that of alamethicin. We conclude that, among naturally produced peptides that we have investigated, only alamethicin conforms to the barrel-stave model. Other peptides, including magainins, melittin and protegrins, all appear to induce transmembrane pores that conform to the toroidal model in which the lipid monolayer bends continuously through the pore so that the water core is lined by both the peptides and the lipid headgroups.

Biophys J, September 2001, p. 1475-1485, Vol. 81, No. 3
© 2001 by the Biophysical Society   0006-3495/01/09/1475/11  $2.00


This article has been cited by other articles:


Home page
 Biophys. JHome page
P. Schon, A. J. Garcia-Saez, P. Malovrh, K. Bacia, G. Anderluh, and P. Schwille
Equinatoxin II Permeabilizing Activity Depends on the Presence of Sphingomyelin and Lipid Phase Coexistence
Biophys. J., July 15, 2008; 95(2): 691 - 698.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Qian, W. Wang, L. Yang, and H. W. Huang
Structure of the Alamethicin Pore Reconstructed by X-Ray Diffraction Analysis
Biophys. J., May 1, 2008; 94(9): 3512 - 3522.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Mechanism and kinetics of pore formation in membranes by water-soluble amphipathic peptides
PNAS, April 1, 2008; 105(13): 5087 - 5092.


Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Giovambattista, C. F. Lopez, P. J. Rossky, and P. G. Debenedetti
Hydrophobicity of protein surfaces: Separating geometry from chemistry
PNAS, February 19, 2008; 105(7): 2274 - 2279.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Arispe, J. C. Diaz, O. Simakova, and H. B. Pollard
Heart failure drug digitoxin induces calcium uptake into cells by forming transmembrane calcium channels
PNAS, February 19, 2008; 105(7): 2610 - 2615.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Mechler, S. Praporski, K. Atmuri, M. Boland, F. Separovic, and L. L. Martin
Specific and Selective Peptide-Membrane Interactions Revealed Using Quartz Crystal Microbalance
Biophys. J., December 1, 2007; 93(11): 3907 - 3916.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
X. Chen, J. Wang, C. B. Kristalyn, and Z. Chen
Real-Time Structural Investigation of a Lipid Bilayer during Its Interaction with Melittin Using Sum Frequency Generation Vibrational Spectroscopy
Biophys. J., August 1, 2007; 93(3): 866 - 875.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. J. Garcia-Saez, S. Chiantia, J. Salgado, and P. Schwille
Pore Formation by a Bax-Derived Peptide: Effect on the Line Tension of the Membrane Probed by AFM
Biophys. J., July 1, 2007; 93(1): 103 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
G. van den Bogaart, J. T. Mika, V. Krasnikov, and B. Poolman
The Lipid Dependence of Melittin Action Investigated by Dual-Color Fluorescence Burst Analysis
Biophys. J., July 1, 2007; 93(1): 154 - 163.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P.-A. Boucher, B. Joos, M. J. Zuckermann, and L. Fournier
Pore Formation in a Lipid Bilayer under a Tension Ramp: Modeling the Distribution of Rupture Tensions
Biophys. J., June 15, 2007; 92(12): 4344 - 4355.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
H. Raghuraman and A. Chattopadhyay
Orientation and Dynamics of Melittin in Membranes of Varying Composition Utilizing NBD Fluorescence
Biophys. J., February 15, 2007; 92(4): 1271 - 1283.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Munoz, B. Lopez-Garcia, and J. F. Marcos
Studies on the Mode of Action of the Antifungal Hexapeptide PAF26
Antimicrob. Agents Chemother., November 1, 2006; 50(11): 3847 - 3855.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. Li and T. Salditt
Structure of Magainin and Alamethicin in Model Membranes Studied by X-Ray Reflectivity
Biophys. J., November 1, 2006; 91(9): 3285 - 3300.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Kozlov, A. A. Vassilevski, A. V. Feofanov, A. Y. Surovoy, D. V. Karpunin, and E. V. Grishin
Latarcins, Antimicrobial and Cytolytic Peptides from the Venom of the Spider Lachesana tarabaevi (Zodariidae) That Exemplify Biomolecular Diversity
J. Biol. Chem., July 28, 2006; 281(30): 20983 - 20992.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
W. Wang, D. Pan, Y. Song, W. Liu, L. Yang, and H. W. Huang
Method of X-Ray Anomalous Diffraction for Lipid Structures
Biophys. J., July 15, 2006; 91(2): 736 - 743.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
F. Neville, M. Cahuzac, O. Konovalov, Y. Ishitsuka, K. Y. C. Lee, I. Kuzmenko, G. M. Kale, and D. Gidalevitz
Lipid Headgroup Discrimination by Antimicrobial Peptide LL-37: Insight into Mechanism of Action
Biophys. J., February 15, 2006; 90(4): 1275 - 1287.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
R. Carmieli, N. Papo, H. Zimmermann, A. Potapov, Y. Shai, and D. Goldfarb
Utilizing ESEEM Spectroscopy to Locate the Position of Specific Regions of Membrane-Active Peptides within Model Membranes
Biophys. J., January 15, 2006; 90(2): 492 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M.-T. Lee, W.-C. Hung, F.-Y. Chen, and H. W. Huang
Many-Body Effect of Antimicrobial Peptides: On the Correlation Between Lipid's Spontaneous Curvature and Pore Formation
Biophys. J., December 1, 2005; 89(6): 4006 - 4016.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Mecke, D.-K. Lee, A. Ramamoorthy, B. G. Orr, and M. M. Banaszak Holl
Membrane Thinning Due to Antimicrobial Peptide Binding: An Atomic Force Microscopy Study of MSI-78 in Lipid Bilayers
Biophys. J., December 1, 2005; 89(6): 4043 - 4050.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Toraya, T. Nagao, K. Norisada, S. Tuzi, H. Saito, S. Izumi, and A. Naito
Morphological Behavior of Lipid Bilayers Induced by Melittin near the Phase Transition Temperature
Biophys. J., November 1, 2005; 89(5): 3214 - 3222.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Meincken, D. L. Holroyd, and M. Rautenbach
Atomic Force Microscopy Study of the Effect of Antimicrobial Peptides on the Cell Envelope of Escherichia coli
Antimicrob. Agents Chemother., October 1, 2005; 49(10): 4085 - 4092.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. C. Dave, E. Billington, Y.-L. Pan, and S. K. Straus
Interaction of Alamethicin with Ether-Linked Phospholipid Bilayers: Oriented Circular Dichroism, 31P Solid-State NMR, and Differential Scanning Calorimetry Studies
Biophys. J., October 1, 2005; 89(4): 2434 - 2442.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. F. Lopez, S. O. Nielsen, B. Ensing, P. B. Moore, and M. L. Klein
Structure and Dynamics of Model Pore Insertion into a Membrane
Biophys. J., May 1, 2005; 88(5): 3083 - 3094.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. Mazzuca, L. Stella, M. Venanzi, F. Formaggio, C. Toniolo, and B. Pispisa
Mechanism of Membrane Activity of the Antibiotic Trichogin GA IV: A Two-State Transition Controlled by Peptide Concentration
Biophys. J., May 1, 2005; 88(5): 3411 - 3421.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. Allende, S. A. Simon, and T. J. McIntosh
Melittin-Induced Bilayer Leakage Depends on Lipid Material Properties: Evidence for Toroidal Pores
Biophys. J., March 1, 2005; 88(3): 1828 - 1837.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kristan, Z. Podlesek, V. Hojnik, I. Gutierrez-Aguirre, G. Guncar, D. Turk, J. M. Gonzalez-Manas, J. H. Lakey, P. Macek, and G. Anderluh
Pore Formation by Equinatoxin, a Eukaryotic Pore-forming Toxin, Requires a Flexible N-terminal Region and a Stable {beta}-Sandwich
J. Biol. Chem., November 5, 2004; 279(45): 46509 - 46517.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Toraya, K. Nishimura, and A. Naito
Dynamic Structure of Vesicle-Bound Melittin in a Variety of Lipid Chain Lengths by Solid-State NMR
Biophys. J., November 1, 2004; 87(5): 3323 - 3335.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
H. Raghuraman and A. Chattopadhyay
Interaction of Melittin with Membrane Cholesterol: A Fluorescence Approach
Biophys. J., October 1, 2004; 87(4): 2419 - 2432.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Barlic, I. Gutierrez-Aguirre, J. M. M. Caaveiro, A. Cruz, M.-B. Ruiz-Arguello, J. Perez-Gil, and J. M. Gonzalez-Manas
Lipid Phase Coexistence Favors Membrane Insertion of Equinatoxin-II, a Pore-forming Toxin from Actinia equina
J. Biol. Chem., August 13, 2004; 279(33): 34209 - 34216.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
O. Toke, R. D. O'Connor, T. K. Weldeghiorghis, W. L. Maloy, R. W. Glaser, A. S. Ulrich, and J. Schaefer
Structure of (KIAGKIA)3 Aggregates in Phospholipid Bilayers by Solid-State NMR
Biophys. J., July 1, 2004; 87(1): 675 - 687.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. F. Lopez, S. O. Nielsen, P. B. Moore, and M. L. Klein
From the Cover: Understanding nature's design for a nanosyringe
PNAS, March 30, 2004; 101(13): 4431 - 4434.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Stella, C. Mazzuca, M. Venanzi, A. Palleschi, M. Didone, F. Formaggio, C. Toniolo, and B. Pispisa
Aggregation and Water-Membrane Partition as Major Determinants of the Activity of the Antibiotic Peptide Trichogin GA IV
Biophys. J., February 1, 2004; 86(2): 936 - 945.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Anderluh, M. D. Serra, G. Viero, G. Guella, P. Macek, and G. Menestrina
Pore Formation by Equinatoxin II, a Eukaryotic Protein Toxin, Occurs by Induction of Nonlamellar Lipid Structures
J. Biol. Chem., November 14, 2003; 278(46): 45216 - 45223.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
C. Tian, P. F. Gao, L. H. Pinto, R. A. Lamb, and T. A. Cross
Initial structural and dynamic characterization of the M2 protein transmembrane and amphipathic helices in lipid bilayers
Protein Sci., November 1, 2003; 12(11): 2597 - 2605.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
P. K. Kienker, K. S. Jakes, R. O. Blaustein, C. Miller, and A. Finkelstein
Sizing the Protein Translocation Pathway of Colicin Ia Channels
J. Gen. Physiol., July 28, 2003; 122(2): 161 - 176.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Malovrh, G. Viero, M. D. Serra, Z. Podlesek, J. H. Lakey, P. Macek, G. Menestrina, and G. Anderluh
A Novel Mechanism of Pore Formation: MEMBRANE PENETRATION BY THE N-TERMINAL AMPHIPATHIC REGION OF EQUINATOXIN
J. Biol. Chem., June 13, 2003; 278(25): 22678 - 22685.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
F.-Y. Chen, M.-T. Lee, and H. W. Huang
Evidence for Membrane Thinning Effect as the Mechanism for Peptide-Induced Pore Formation
Biophys. J., June 1, 2003; 84(6): 3751 - 3758.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Zemel, D. R. Fattal, and A. Ben-Shaul
Energetics and Self-Assembly of Amphipathic Peptide Pores in Lipid Membranes
Biophys. J., April 1, 2003; 84(4): 2242 - 2255.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
B. B. Bonev, Y.-H. Lam, G. Anderluh, A. Watts, R. S. Norton, and F. Separovic
Effects of the Eukaryotic Pore-Forming Cytolysin Equinatoxin II on Lipid Membranes and the Role of Sphingomyelin
Biophys. J., April 1, 2003; 84(4): 2382 - 2392.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
M. R. Yeaman and N. Y. Yount
Mechanisms of Antimicrobial Peptide Action and Resistance
Pharmacol. Rev., March 1, 2003; 55(1): 27 - 55.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
T. Jacobs, H. Bruhn, I. Gaworski, B. Fleischer, and M. Leippe
NK-Lysin and Its Shortened Analog NK-2 Exhibit Potent Activities against Trypanosoma cruzi
Antimicrob. Agents Chemother., February 1, 2003; 47(2): 607 - 613.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
T. M. Weiss, P. C. A. van der Wel, J. A. Killian, R. E. Koeppe II, and H. W. Huang
Hydrophobic Mismatch between Helices and Lipid Bilayers
Biophys. J., January 1, 2003; 84(1): 379 - 385.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Basanez, J. C. Sharpe, J. Galanis, T. B. Brandt, J. M. Hardwick, and J. Zimmerberg
Bax-type Apoptotic Proteins Porate Pure Lipid Bilayers through a Mechanism Sensitive to Intrinsic Monolayer Curvature
J. Biol. Chem., December 13, 2002; 277(51): 49360 - 49365.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Biophysical Society.