Biophys J, November 2001, p. 2458-2472, Vol. 81, No. 5

Insertion and Pore Formation Driven by Adsorption of Proteins Onto Lipid Bilayer Membrane-Water Interfaces

Martin J. Zuckermann^* and Thomas Heimburg^*

 ^*MEMPHYS Group, Department of Chemistry, Technical University of Denmark, DK-2800 Lyngby, Denmark,  School of Physics, University of New South Wales, Sydney 2052, Australia, and  Membrane Thermodynamics Group, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany

We describe the binding of proteins to lipid bilayers in the case for which binding can occur either by adsorption to the lipid bilayer membrane-water interface or by direct insertion into the bilayer itself. We examine in particular the case when the insertion and pore formation are driven by the adsorption process using scaled particle theory. The adsorbed proteins form a two-dimensional "surface gas" at the lipid bilayer membrane-water interface that exerts a lateral pressure on the lipid bilayer membrane. Under conditions of strong intrinsic binding and a high degree of interfacial converge, this pressure can become high enough to overcome the energy barrier for protein insertion. Under these conditions, a subtle equilibrium exists between the adsorbed and inserted proteins. We propose that this provides a control mechanism for reversible insertion and pore formation of proteins such as melittin and magainin. Next, we discuss experimental data for the binding isotherms of cytochrome c to charged lipid membranes in the light of our theory and predict that cytochrome c inserts into charged lipid bilayers at low ionic strength. This prediction is supported by titration calorimetry results that are reported here. We were furthermore able to describe the observed binding isotherms of the pore-forming peptides endotoxin (5-helix) and of pardaxin to zwitterionic vesicles from our theory by assuming adsorption/insertion equilibrium.

Biophys J, November 2001, p. 2458-2472, Vol. 81, No. 5
© 2001 by the Biophysical Society   0006-3495/01/11/2458/15  $2.00

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