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Biophys J, November 2001, p. 2639-2646, Vol. 81, No. 5
and
Departments of *Biophysics and Biology, Leiden
University, 2333 CA Leiden, The Netherlands, Institute
for Biophysics, University of Linz, 4040 Linz, Austria, and
§National Institute on Aging, National Institutes of
Health, Baltimore, Maryland 21224, USA
L-type Ca2+ channels are an important means
by which a cell regulates the Ca2+ influx into the cytosol
on electrical stimulation. Their structure and dynamics in the plasma
membrane, including their molecular mobility and aggregation, is of key
interest for the in-depth understanding of their function. Construction
of a fluorescent variant by fusion of the yellow-fluorescent protein to
the ion channel and expression in a human cell line allowed us to
address its dynamic embedding in the membrane at the level of
individual channels in vivo. We report on the observation of individual
fluorescence-labeled human cardiac L-type Ca2+ channels
using wide-field fluorescence microscopy in living cells. Our
fluorescence and electrophysiological data indicate that L-type Ca2+ channels tend to form larger aggregates which are
mobile in the plasma membrane.
Biophys J, November 2001, p. 2639-2646, Vol. 81, No. 5
© 2001 by the Biophysical Society 0006-3495/01/11/2639/08 $2.00
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