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Biophys J, November 2001, p. 2743-2751, Vol. 81, No. 5

Kinetics of Membrane Adhesion Mediated by Ligand-Receptor Interaction Studied with a Biomimetic System

Alexei Boulbitch, Zeno Guttenberg, and Erich Sackmann

Department für Biophysik E22, Technische Universität München, D-85747 Garching bei München, Germany

We report the first measurement of the kinetics of adhesion of a single giant vesicle controlled by the competition between membrane-substrate interaction mediated by ligand-receptor interaction, gravitation, and Helfrich repulsion. To model the cell-tissue interaction, we doped the vesicles with lipid-coupled polymers (mimicking the glycocalix) and the reconstituted ligands selectively recognized by alpha IIbbeta 3 integrin-mediating specific attraction forces. The integrin was grafted on glass substrates to act as a target cell. The adhesion of the vesicle membrane to the integrin-covered surface starts with the spontaneous formation of a small (~200 nm) domain of tight adhesion, which then gradually grows until the whole adhesion area is in the state of tight adhesion. The time of adhesion varies from few tens of seconds to about one hour depending on the ligand and lipopolymer concentration. At small ligand concentrations, we observed the displacement xi  of the front of tight adhesion following the square root law xi  ~ t1/2, whereas, at high concentrations, we found a linear law xi  ~ t. We show both experimentally and theoretically that the t1/2-regime is dominated by diffusion of ligands, and the xi  ~ t-regime by the kinetics of ligands-receptors association.

Biophys J, November 2001, p. 2743-2751, Vol. 81, No. 5
© 2001 by the Biophysical Society   0006-3495/01/11/2743/09  $2.00


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