help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rostovtseva, T. K.
Right arrow Articles by Colombini, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rostovtseva, T. K.
Right arrow Articles by Colombini, M.

Biophys J, January 2002, p. 193-205, Vol. 82, No. 1

Dynamics of Nucleotides in VDAC Channels: Structure-Specific Noise Generation

Tatiana K. Rostovtseva,* Alexander Komarov,dagger Dagger Sergey M. Bezrukov,dagger Dagger and Marco Colombini*

 *Department of Biology, University of Maryland, College Park, Maryland 20742,  dagger Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 USA, and  Dagger St. Petersburg Nuclear Physics Institute, Gatchina 188350, Russia

Nucleotide penetration into the voltage-dependent mitochondrial ion channel (VDAC) reduces single-channel conductance and generates excess current noise through a fully open channel. VDAC channels were reconstituted into planar phospholipid membranes bathed in 1.0 M NaCl. At a given nucleotide concentration, the average decrease in small-ion channel conductance induced by mononucleotides ATP, ADP, AMP, and UTP and dinucleotides beta - and alpha -NADH, NAD, and NADPH are very close. However, the excess current noise is about seven times higher in the presence of NADPH than in the presence of ATP and is about 40 times higher than in the presence of UTP. The nucleotide-generated low-frequency noise obeys the following sequence: beta -NADPH > beta -NADH = alpha -NADH > ATP > ADP > beta -NAD >=  AMP > UTP. Measurements of bulk-phase diffusion coefficients and of the effective charge of the nucleotides in 1.0 M NaCl suggest that differences in size and charge cannot be the major factors responsible for the ability to generate current noise. Thus, although the ability of nucleotides to partition into the channel's pore, as assessed by the reduction in conductance, is very similar, the ability to generate current noise involves a detailed recognition of the three-dimensional structure of the nucleotide by the VDAC channel. A possible mechanism for this selectivity is two noise-generating processes operating in parallel.

Biophys J, January 2002, p. 193-205, Vol. 82, No. 1
© 2002 by the Biophysical Society   0006-3495/02/01/193/13  $2.00


This article has been cited by other articles:


Home page
 Biophys. JHome page
W. Tan, Y.-H. Loke, C. A. Stein, P. Miller, and M. Colombini
Phosphorothioate Oligonucleotides Block the VDAC Channel
Biophys. J., August 15, 2007; 93(4): 1184 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Yehezkel, N. Hadad, H. Zaid, S. Sivan, and V. Shoshan-Barmatz
Nucleotide-binding Sites in the Voltage-dependent Anion Channel: CHARACTERIZATION AND LOCALIZATION
J. Biol. Chem., March 3, 2006; 281(9): 5938 - 5946.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. G. Komarov, D. Deng, W. J. Craigen, and M. Colombini
New Insights into the Mechanism of Permeation through Large Channels
Biophys. J., December 1, 2005; 89(6): 3950 - 3959.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Guo, D. Pietkiewicz, E. V. Pavlov, S. M. Grigoriev, J. J. Kasianowicz, L. M. Dejean, S. J. Korsmeyer, B. Antonsson, and K. W. Kinnally
Effects of cytochrome c on the mitochondrial apoptosis-induced channel MAC
Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1109 - C1117.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. G. Komarov, B. H. Graham, W. J. Craigen, and M. Colombini
The Physiological Properties of a Novel Family of VDAC-Like Proteins from Drosophila melanogaster
Biophys. J., January 1, 2004; 86(1): 152 - 162.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. Muro, S. M. Grigoriev, D. Pietkiewicz, K. W. Kinnally, and M. L. Campo
Comparison of the TIM and TOM Channel Activities of the Mitochondrial Protein Import Complexes
Biophys. J., May 1, 2003; 84(5): 2981 - 2989.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Biophysical Society.