help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baker, H. L.
Right arrow Articles by Campbell, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baker, H. L.
Right arrow Articles by Campbell, A. K.

Biophys J, February 2002, p. 582-590, Vol. 82, No. 2

A Mathematical Model Predicts that Calreticulin Interacts with the Endoplasmic Reticulum Ca2+-ATPase

Helen L. Baker, Rachel J. Errington, Sally C. Davies, and Anthony K. Campbell

Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, United Kingdom

A robust mathematical model developed from single cell calcium (Ca2+) dynamics has enabled us to predict the consequences of over-expression of endoplasmic reticulum-located chaperones. Model predictions concluded that calreticulin interacts with the lumenal domain of the sarcoplasmic and endoplasmic reticulum Ca2+-activated ATPase (SERCA) pump, altering pump affinity for Ca2+ (K1/2 switches from 247 to 431 nM) and hence generating Ca2+ oscillations. Expression of calreticulin in the ER generated an average of six transient-decline oscillations during the Ca2+ recovery phase, upon exposure to maximal levels of the agonist ATP. In contrast, normal cells produced a single Ca2+ transient with few or no oscillations. By conditioning the model to experimental data, parameters for generation and decay of IP3 and SERCA pump kinetics were determined. To elucidate the possible source of the oscillatory behavior three possible oscillators, 1) IP3, 2) IP3R, and 3) SERCA pump, were investigated and parameters constrained by experimental data to produce the best candidate. Each of the three oscillators generated very good fits with experimental data. However, converting a normal exponential recovery to a transient-decline oscillator predicted that the SERCA pump is the most likely candidate for calreticulin-meditated Ca2+ release, highlighting the role of this chaperone as a signal protein within the endoplasmic reticulum.

Biophys J, February 2002, p. 582-590, Vol. 82, No. 2
© 2002 by the Biophysical Society   0006-3495/02/02/582/09  $2.00


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Hong, D. Jaron, D. G. Buerk, and K. A. Barbee
Transport-dependent calcium signaling in spatially segregated cellular caveolar domains
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C856 - C866.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
V. Becker, H. Gonzalez-Serratos, R. Alvarez, M. Baermann, C. Irles, and A. Ortega
Effect of endurance exercise on the Ca2+ pumps from transverse tubule and sarcoplasmic reticulum of rabbit skeletal muscle
J Appl Physiol, August 1, 2004; 97(2): 467 - 474.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Papp, E. Dziak, M. Michalak, and M. Opas
Is all of the endoplasmic reticulum created equal? The effects of the heterogeneous distribution of endoplasmic reticulum Ca2+-handling proteins
J. Cell Biol., February 18, 2003; 160(4): 475 - 479.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Biophysical Society.